These primers are used to verify the integration of the marker cassette. Default primers are provided for KanMX6 and NatMX6 cassettes, but you can specify your own primers if using a different marker.
Should align with the top strand of the resulting allele, and will produce a PCR product with a primer that aligns with the bottom strand on the right side of the insertion.
Should align with the bottom strand of the resulting allele, and will produce a PCR product with a primer that aligns with the top strand on the left side of the insertion.