{% macro text(cutoff) %}
Sambamba {{ _('is used for coverage analysis based on read alignment.') }} {{ _('Duplicates, reads with mapping quality less than 10 as well as bases with a base quality less than 10 are not included in the analysis.') }} {{ _('Bases that overlap between read pairs are only counted once.') }} {{ _('The parts of the genome that has been analyzed') }} {{ _('is composed of all protein coding, exonic intervals') }} {{ _('referenced in the') }} {{ _('CCDS database') }}. {% if includes_splice_sites %} {{ _('Additionally, the data also includes spicing sites') }} ({{ _('donator and acceptor positions') }}, {{ splice_site_interval }} {{ _('bases on either of each exon') }}). {% endif %}
{% if config.CHANJO_PANEL %}
{{ _('Final coverage metrics were calculated for genes from the clinical panels') }}:
{{ config.CHANJO_PANEL_NAME }}
.
{{ _('Note that estimations were made regarding coverage and completeness') }}
{{ _('on the level of transcripts') }}.
{{ _('Completeness') }}: {{ _('defined as the ratio of bases') }} {{ _('sequenced deeper than a specified cutoff') }}, {{ _('e.g. 10x') }}.
{{ _('Transcript coverage') }}: {{ _('defined as the ratio of transcripts that are fully covered') }}, {{ _('i.e. with a completeness of') }} 100% {{ _('at') }} {{ cutoff }}x.
{{ _('Gender prediction from data') }}: {{ _('calculated by comparing relative chromosome coverage') }} (X/Y). {{ _('Some reads randomly map to the Y chromosome') }}. {{ _('Therefore, a less than 10 fold difference is used to infer a "male" sample') }}.
{% endmacro %}