Metadata
| Filename | 21C125_R1.fastq.gz |
| Filesize | 0.45 GiB |
| Filename read 2 | 21C125_R2.fastq.gz |
| Filesize read 2 | 0.46 GiB |
| Sequali version | 0.10.1.dev10+g545d2be |
| Report generated on | 2024-06-10 06:33:03+0200 |
Read 1: Summary
| Mean length | 151.00 | |
| Length range (min-max) | 151 | 151 |
| Total reads | 5,477,353 | |
| Q20 reads | 5,214,420 | 95.20% |
| Total bases | 827,080,303 | |
| Total GC bases | 273,860,586 | 33.11% |
| Q20 bases | 798,680,482 | 96.57% |
Read 2: Summary
| Mean length | 151.00 | |
| Length range (min-max) | 151 | 151 |
| Total reads | 5,477,353 | |
| Q20 reads | 5,243,046 | 95.72% |
| Total bases | 827,080,303 | |
| Total GC bases | 273,545,195 | 33.07% |
| Q20 bases | 797,522,582 | 96.43% |
Read 1: Sequence length distribution
Read 2: Sequence length distribution
Read 1: Per position quality score distribution
Read 2: Per position quality score distribution
Read 1: Per position quality percentiles
Shows the mean for all bases and the means of the lowest and highest percentiles to indicate the spread. Since the graph is based on the sampled categories, rather than exact phreds, it is an approximation.
Read 2: Per position quality percentiles
Shows the mean for all bases and the means of the lowest and highest percentiles to indicate the spread. Since the graph is based on the sampled categories, rather than exact phreds, it is an approximation.
Read 1: Per sequence average quality scores
| >=Q5 | 5,477,353 | 100.00% |
| >=Q7 | 5,477,353 | 100.00% |
| >=Q10 | 5,477,330 | 100.00% |
| >=Q12 | 5,477,310 | 100.00% |
| >=Q15 | 5,473,870 | 99.94% |
| >=Q20 | 5,214,420 | 95.20% |
| >=Q30 | 1,971,310 | 35.99% |
Read 2: Per sequence average quality scores
| >=Q5 | 5,477,353 | 100.00% |
| >=Q7 | 5,477,350 | 100.00% |
| >=Q10 | 5,477,301 | 100.00% |
| >=Q12 | 5,477,227 | 100.00% |
| >=Q15 | 5,466,968 | 99.81% |
| >=Q20 | 5,243,046 | 95.72% |
| >=Q30 | 1,549,324 | 28.29% |
Read 1: Per position base content
Read 2: Per position base content
Read 1: Per position N content
Read 2: Per position N content
Read 1: Per sequence GC content
For short reads with fixed size (i.e. Illumina) the plot will look very spiky due to the GC content calculation: GC bases / all bases. For read lengths of 151, both 75 and 76 GC bases lead to a percentage of 50% (rounded) and 72 and 73 GC bases leads to 48% (rounded). Only 74 GC bases leads to 49%. As a result the even categories will be twice as high, which creates a spike. The smoothened plot is provided to give a clearer picture in this case.
Read 2: Per sequence GC content
For short reads with fixed size (i.e. Illumina) the plot will look very spiky due to the GC content calculation: GC bases / all bases. For read lengths of 151, both 75 and 76 GC bases lead to a percentage of 50% (rounded) and 72 and 73 GC bases leads to 48% (rounded). Only 74 GC bases leads to 49%. As a result the even categories will be twice as high, which creates a spike. The smoothened plot is provided to give a clearer picture in this case.
Adapter Content
By calculating the overlap between reads it is possible to determine where the adapter is.
| Total reads | 5,477,353 | 100.00% |
| Adapters in read 1 | 1,834,674 | 33.50% |
| Adapters in read 2 | 1,834,674 | 33.50% |
| Longest most frequent adapter | Adapter Sequence | Best match |
|---|---|---|
| Read 1 | CTGTCTCTTATACACATCTCCGAGCCCACGA | gnl|uv|NGB00726.1:1-34 Illumina Nextera transposase sequence Read 2 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| Read 2 | CTGTCTCTTATACACATCTGACGCTGCCGAC | gnl|uv|NGB00725.1:1-33 Illumina Nextera transposase sequence Read 1 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
Adapters read 1
| Adapter | Count |
|---|---|
| C | 21908 |
| CT | 22392 |
| CTG | 22566 |
| CTGT | 22406 |
| CTGTC | 22114 |
| CTGTCT | 22459 |
| CTGTCTC | 22067 |
| CTGTCTCT | 22882 |
| CTGTCTCTT | 22742 |
| CTGTCTCTTA | 22790 |
| CTGTCTCTTAT | 22434 |
| CTGTCTCTTATA | 22582 |
| CTGTCTCTTATAC | 22132 |
| CTGTCTCTTATACA | 22417 |
| CTGTCTCTTATACAC | 22842 |
| CTGTCTCTTATACACA | 22801 |
| CTGTCTCTTATACACAT | 22547 |
| CTGTCTCTTATACACATC | 22387 |
| CTGTCTCTTATACACATCT | 22785 |
| CTGTCTCTTATACACATCTC | 22647 |
| CTGTCTCTTATACACATCTCC | 22655 |
| CTGTCTCTTATACACATCTCCG | 21916 |
| CTGTCTCTTATACACATCTCCGA | 21661 |
| CTGTCTCTTATACACATCTCCGAG | 22071 |
| CTGTCTCTTATACACATCTCCGAGC | 22229 |
| CTGTCTCTTATACACATCTCCGAGCC | 22234 |
| CTGTCTCTTATACACATCTCCGAGCCC | 22728 |
| CTGTCTCTTATACACATCTCCGAGCCCA | 21942 |
| CTGTCTCTTATACACATCTCCGAGCCCAC | 22272 |
| CTGTCTCTTATACACATCTCCGAGCCCACG | 22093 |
| CTGTCTCTTATACACATCTCCGAGCCCACGA | 1049249 |
Adapters read 2
| Adapter | Count |
|---|---|
| C | 21351 |
| CT | 22003 |
| CTG | 22202 |
| CTGT | 22198 |
| CTGTC | 21694 |
| CTGTCT | 22182 |
| CTGTCTC | 21657 |
| CTGTCTCT | 22528 |
| CTGTCTCTT | 22440 |
| CTGTCTCTTA | 22510 |
| CTGTCTCTTAT | 22274 |
| CTGTCTCTTATA | 22421 |
| CTGTCTCTTATAC | 21408 |
| CTGTCTCTTATACA | 21996 |
| CTGTCTCTTATACAC | 22437 |
| CTGTCTCTTATACACA | 22327 |
| CTGTCTCTTATACACAT | 22121 |
| CTGTCTCTTATACACATC | 22027 |
| CTGTCTCTTATACACATCT | 22470 |
| CTGTCTCTTATACACATCTG | 22118 |
| CTGTCTCTTATACACATCTGA | 22460 |
| CTGTCTCTTATACACATCTGAC | 22209 |
| CTGTCTCTTATACACATCTGACG | 21792 |
| CTGTCTCTTATACACATCTGACGC | 21918 |
| CTGTCTCTTATACACATCTGACGCT | 22235 |
| CTGTCTCTTATACACATCTGACGCTG | 22269 |
| CTGTCTCTTATACACATCTGACGCTGC | 22964 |
| CTGTCTCTTATACACATCTGACGCTGCC | 22048 |
| CTGTCTCTTATACACATCTGACGCTGCCG | 22280 |
| CTGTCTCTTATACACATCTGACGCTGCCGA | 22053 |
| CTGTCTCTTATACACATCTGACGCTGCCGAC | 1063178 |
Insert Sizes
Insert sizes are calculated by taking the first and last 16 bp from read 2. These are searched for in read 1 while allowing at most 1 error. This method is very fast and reasonably accurate, but does not account for long poly-G cycles.
| Total reads | 5,477,353 | |
| Reads without overlap | 1,552,343 | 28.34% |
Read 1: Per tile quality
This graph shows the deviation of each tile on each position from the geometric mean of all tiles at that position. The scale is expressed in phred units. -10 is 10 times more errors than the average. -3 is ~2 times more errors than the average. Only points that deviate more than 2 phred units from the average are shown.
Tiles with more than 2 times the average error: 1142, 1549, 1569, 2113, 2136, 2150, 2231, 2256, 2264, 2273, 2548, 2550, 2619, 2671, 2676
Tiles with more than 10 times the average error: 2114, 2610, 2611, 2617, 2618
Read 2: Per tile quality
This graph shows the deviation of each tile on each position from the geometric mean of all tiles at that position. The scale is expressed in phred units. -10 is 10 times more errors than the average. -3 is ~2 times more errors than the average. Only points that deviate more than 2 phred units from the average are shown.
Tiles with more than 2 times the average error: 1444, 1540, 2278, 2373, 2669
Tiles with more than 10 times the average error:
Duplication percentages
Fingerprints are taken by taking a sample from the beginning and the end at an offset. The samples are combined and hashed while using the length as a seed. A subsample of these fingerprints is stored to estimate the duplication rate. See the documentation for a complete explanation.
| Fingerprint front sequence length | 8 |
| Fingerprint front sequence offset | 0 |
| Fingerprint back sequence length | 8 |
| Fingerprint back sequence offset | 0 |
| Subsampled fingerprints | 572,140 |
| Estimated remaining sequences if deduplicated | 83.47% |
Read 1: Overrepresented sequences
A subsample of the sequences is analysed Sequences are cut into fragments of up to 31 bp. Fragments are stored and counted. When the maximum amount of unique fragments is reached, only fragments that are already stored are counted. The rest of the fragments is ignored. Fragments are stored in their canonical representation. That is either the sequence or the reverse complement, whichever has the lowest sort order. Both representations are shown in the table. See the documentation for a complete explanation. The sequence identity is calculated by .
The percentage shown is an estimate based on the number of occurences of the fragment in relation to the number of sampled sequences. Fragments are only counted once per sequence. Fragments that occur more than once in a sequence are counted as one.
| Total sequences in file | 5,477,353 |
| Sampled sequences | 684,670 |
| Sampling rate | 1 in 8 |
| Total fragments sampled | 5,475,263 |
| Stored unique fragments | 1,828,940 |
| Maximum unique fragments | 5,000,000 |
| Fragment size | 21 |
| count | percentage | canonical sequence | reverse complemented sequence | sequence identity | best match |
|---|---|---|---|---|---|
| 9890 | 1.44% | CTGTCTCTTATACACATCTCC | GGAGATGTGTATAAGAGACAG | 100.00% | gnl|uv|NGB00726.1:1-34 Illumina Nextera transposase sequence Read 2 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 9597 | 1.40% | CGGAGATGTGTATAAGAGACA | TGTCTCTTATACACATCTCCG | 100.00% | gnl|uv|NGB00726.1:1-34 Illumina Nextera transposase sequence Read 2 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 9317 | 1.36% | GTCTCTTATACACATCTCCGA | TCGGAGATGTGTATAAGAGAC | 100.00% | gnl|uv|NGB00726.1:1-34 Illumina Nextera transposase sequence Read 2 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 9240 | 1.35% | CTCTTATACACATCTCCGAGC | GCTCGGAGATGTGTATAAGAG | 100.00% | gnl|uv|NGB00726.1:1-34 Illumina Nextera transposase sequence Read 2 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 9239 | 1.35% | CTCGGAGATGTGTATAAGAGA | TCTCTTATACACATCTCCGAG | 100.00% | gnl|uv|NGB00726.1:1-34 Illumina Nextera transposase sequence Read 2 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 8908 | 1.30% | CTTATACACATCTCCGAGCCC | GGGCTCGGAGATGTGTATAAG | 100.00% | gnl|uv|NGB00726.1:1-34 Illumina Nextera transposase sequence Read 2 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 8905 | 1.30% | GGCTCGGAGATGTGTATAAGA | TCTTATACACATCTCCGAGCC | 100.00% | gnl|uv|NGB00726.1:1-34 Illumina Nextera transposase sequence Read 2 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 8597 | 1.26% | TGGGCTCGGAGATGTGTATAA | TTATACACATCTCCGAGCCCA | 100.00% | gnl|uv|NGB00726.1:1-34 Illumina Nextera transposase sequence Read 2 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 8394 | 1.23% | GTGGGCTCGGAGATGTGTATA | TATACACATCTCCGAGCCCAC | 100.00% | gnl|uv|NGB00726.1:1-34 Illumina Nextera transposase sequence Read 2 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 8230 | 1.20% | ATACACATCTCCGAGCCCACG | CGTGGGCTCGGAGATGTGTAT | 100.00% | gnl|uv|NGB00726.1:1-34 Illumina Nextera transposase sequence Read 2 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 7983 | 1.17% | TACACATCTCCGAGCCCACGA | TCGTGGGCTCGGAGATGTGTA | 100.00% | gnl|uv|NGB00726.1:1-34 Illumina Nextera transposase sequence Read 2 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 7841 | 1.15% | ACACATCTCCGAGCCCACGAG | CTCGTGGGCTCGGAGATGTGT | 100.00% | gnl|uv|NGB00726.1:1-34 Illumina Nextera transposase sequence Read 2 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 7650 | 1.12% | CACATCTCCGAGCCCACGAGA | TCTCGTGGGCTCGGAGATGTG | 100.00% | gnl|uv|NGB00726.1:1-34 Illumina Nextera transposase sequence Read 2 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 7463 | 1.09% | ACATCTCCGAGCCCACGAGAC | GTCTCGTGGGCTCGGAGATGT | 100.00% | gnl|uv|NGB00726.1:1-34 Illumina Nextera transposase sequence Read 2 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 7425 | 1.08% | AGTCTCGTGGGCTCGGAGATG | CATCTCCGAGCCCACGAGACT | 95.24% | gnl|uv|NGB00726.1:1-34 Illumina Nextera transposase sequence Read 2 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 7205 | 1.05% | AAGTCTCGTGGGCTCGGAGAT | ATCTCCGAGCCCACGAGACTT | 90.48% | gnl|uv|NGB00726.1:1-34 Illumina Nextera transposase sequence Read 2 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 7087 | 1.04% | CAAGTCTCGTGGGCTCGGAGA | TCTCCGAGCCCACGAGACTTG | 85.71% | gnl|uv|NGB00726.1:1-34 Illumina Nextera transposase sequence Read 2 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 7016 | 1.02% | CACAAGTCTCGTGGGCTCGGA | TCCGAGCCCACGAGACTTGTG | 85.71% | gnl|uv|NGB00740.1:1-47 Illumina Nextera PCR primer i7 index N706 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 6896 | 1.01% | ACAAGTCTCGTGGGCTCGGAG | CTCCGAGCCCACGAGACTTGT | 80.95% | gnl|uv|NGB00726.1:1-34 Illumina Nextera transposase sequence Read 2 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 6765 | 0.99% | CCGAGCCCACGAGACTTGTGA | TCACAAGTCTCGTGGGCTCGG | 90.48% | gnl|uv|NGB00738.1:1-47 Illumina Nextera PCR primer i7 index N704 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 6601 | 0.96% | CGAGCCCACGAGACTTGTGAA | TTCACAAGTCTCGTGGGCTCG | 85.71% | gnl|uv|NGB00738.1:1-47 Illumina Nextera PCR primer i7 index N704 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 6423 | 0.94% | CTTCACAAGTCTCGTGGGCTC | GAGCCCACGAGACTTGTGAAG | 85.71% | gnl|uv|NGB00738.1:1-47 Illumina Nextera PCR primer i7 index N704 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 6255 | 0.91% | AGCCCACGAGACTTGTGAAGG | CCTTCACAAGTCTCGTGGGCT | 80.95% | gnl|uv|NGB00738.1:1-47 Illumina Nextera PCR primer i7 index N704 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 6222 | 0.91% | CCCCCCCCCCCCCCCCCCCCC | GGGGGGGGGGGGGGGGGGGGG | 100.00% | Poly-G. Illumina dark cycle pattern. |
| 6042 | 0.88% | GCCCACGAGACTTGTGAAGGC | GCCTTCACAAGTCTCGTGGGC | 80.95% | gnl|uv|NGB00738.1:1-47 Illumina Nextera PCR primer i7 index N704 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 5900 | 0.86% | CCCACGAGACTTGTGAAGGCA | TGCCTTCACAAGTCTCGTGGG | 80.95% | gnl|uv|U59231.1:5103-7490 Cloning vector cLHYGpk |
| 5692 | 0.83% | ATGCCTTCACAAGTCTCGTGG | CCACGAGACTTGTGAAGGCAT | 80.95% | gnl|uv|U59231.1:5103-7490 Cloning vector cLHYGpk |
| 5446 | 0.80% | CACGAGACTTGTGAAGGCATC | GATGCCTTCACAAGTCTCGTG | 80.95% | gnl|uv|U59231.1:5103-7490 Cloning vector cLHYGpk |
| 3895 | 0.57% | CGAGACTTGTGAAGGCATCTC | GAGATGCCTTCACAAGTCTCG | 80.95% | gnl|uv|U59231.1:5103-7490 Cloning vector cLHYGpk |
| 3719 | 0.54% | CGAGATGCCTTCACAAGTCTC | GAGACTTGTGAAGGCATCTCG | 80.95% | gnl|uv|U59231.1:5103-7490 Cloning vector cLHYGpk |
| 3580 | 0.52% | ACGAGATGCCTTCACAAGTCT | AGACTTGTGAAGGCATCTCGT | 80.95% | gnl|uv|U59231.1:5103-7490 Cloning vector cLHYGpk |
| 3496 | 0.51% | ACGAGACTTGTGAAGGCATCT | AGATGCCTTCACAAGTCTCGT | 76.19% | gnl|uv|U59231.1:5103-7490 Cloning vector cLHYGpk |
| 3378 | 0.49% | CCTGTCTCTTATACACATCTC | GAGATGTGTATAAGAGACAGG | 100.00% | gnl|uv|JN835257.1:94-190 Cloning vector pIGWZMCS |
| 3371 | 0.49% | GACTTGTGAAGGCATCTCGTA | TACGAGATGCCTTCACAAGTC | 80.95% | gnl|uv|U59231.1:5103-7490 Cloning vector cLHYGpk |
| 3287 | 0.48% | ACTTGTGAAGGCATCTCGTAT | ATACGAGATGCCTTCACAAGT | 80.95% | gnl|uv|NGB00366.1:1-43 Illumina Multiplexing PCR Primer Index 3 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 3110 | 0.45% | CATACGAGATGCCTTCACAAG | CTTGTGAAGGCATCTCGTATG | 71.43% | gnl|uv|NGB01090.1:1-66 Rubicon Genomics ThruPLEX DNA-seq dual-index D502 |
| 3024 | 0.44% | GAGATGTGTATAAGAGACAGA | TCTGTCTCTTATACACATCTC | 100.00% | gnl|uv|JN243340.1:723-820 Cloning vector R995 Km Tp |
| 2934 | 0.43% | GCATACGAGATGCCTTCACAA | TTGTGAAGGCATCTCGTATGC | 76.19% | gnl|uv|NGB01090.1:1-66 Rubicon Genomics ThruPLEX DNA-seq dual-index D502 |
| 2876 | 0.42% | GGCATACGAGATGCCTTCACA | TGTGAAGGCATCTCGTATGCC | 80.95% | gnl|uv|NGB01090.1:1-66 Rubicon Genomics ThruPLEX DNA-seq dual-index D502 |
| 2815 | 0.41% | CGGCATACGAGATGCCTTCAC | GTGAAGGCATCTCGTATGCCG | 85.71% | gnl|uv|NGB01090.1:1-66 Rubicon Genomics ThruPLEX DNA-seq dual-index D502 |
| 2510 | 0.37% | ACGGCATACGAGATGCCTTCA | TGAAGGCATCTCGTATGCCGT | 90.48% | gnl|uv|NGB01090.1:1-66 Rubicon Genomics ThruPLEX DNA-seq dual-index D502 |
| 2492 | 0.36% | AAGGCATCTCGTATGCCGTCT | AGACGGCATACGAGATGCCTT | 95.24% | gnl|uv|NGB00366.1:1-43 Illumina Multiplexing PCR Primer Index 3 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 2405 | 0.35% | GAAGGCATCTCGTATGCCGTC | GACGGCATACGAGATGCCTTC | 90.48% | gnl|uv|NGB00366.1:1-43 Illumina Multiplexing PCR Primer Index 3 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 2298 | 0.34% | AAGACGGCATACGAGATGCCT | AGGCATCTCGTATGCCGTCTT | 100.00% | gnl|uv|NGB00366.1:1-43 Illumina Multiplexing PCR Primer Index 3 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 2150 | 0.31% | GAAGACGGCATACGAGATGCC | GGCATCTCGTATGCCGTCTTC | 100.00% | gnl|uv|NGB00366.1:1-43 Illumina Multiplexing PCR Primer Index 3 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 2076 | 0.30% | GAGATGTGTATAAGAGACAGC | GCTGTCTCTTATACACATCTC | 95.24% | gnl|uv|NGB00726.1:1-34 Illumina Nextera transposase sequence Read 2 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 2004 | 0.29% | ACGAGACTTGTGAAGGCATCG | CGATGCCTTCACAAGTCTCGT | 80.95% | gnl|uv|JQ437370.1:1-703 Nanoluc luciferase reporter vector pNL1.1[Nluc] |
| 1903 | 0.28% | AGAAGACGGCATACGAGATGC | GCATCTCGTATGCCGTCTTCT | 100.00% | gnl|uv|NGB00366.1:1-43 Illumina Multiplexing PCR Primer Index 3 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 1902 | 0.28% | CAGAAGACGGCATACGAGATG | CATCTCGTATGCCGTCTTCTG | 100.00% | gnl|uv|NGB00374.1:1-43 Illumina Multiplexing PCR Primer Index 11 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 1703 | 0.25% | ATCTCGTATGCCGTCTTCTGC | GCAGAAGACGGCATACGAGAT | 100.00% | gnl|uv|NGB00380.1:1-26 Illumina Small RNA 3' Adapter (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 1672 | 0.24% | CGTATGCCGTCTTCTGCTTGA | TCAAGCAGAAGACGGCATACG | 100.00% | gnl|uv|JX069761.1:1-102 Cloning vector pFosill-1 |
| 1649 | 0.24% | ACTGTCTCTTATACACATCTC | GAGATGTGTATAAGAGACAGT | 100.00% | gnl|uv|JN243349.1:63-159 Cloning vector R995 Tp |
| 1570 | 0.23% | AGCAGAAGACGGCATACGAGA | TCTCGTATGCCGTCTTCTGCT | 100.00% | gnl|uv|NGB00380.1:1-26 Illumina Small RNA 3' Adapter (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 1532 | 0.22% | AGATGTGTATAAGAGACAGAT | ATCTGTCTCTTATACACATCT | 100.00% | gnl|uv|NGB00399.1:2658-2791 pCDC-1K Cloning Vector |
| 1526 | 0.22% | GTATGCCGTCTTCTGCTTGAA | TTCAAGCAGAAGACGGCATAC | 95.24% | gnl|uv|JX069761.1:1-102 Cloning vector pFosill-1 |
| 1454 | 0.21% | CGAGACTTGTGAAGGCATCGC | GCGATGCCTTCACAAGTCTCG | 80.95% | gnl|uv|JQ437370.1:1-703 Nanoluc luciferase reporter vector pNL1.1[Nluc] |
| 1383 | 0.20% | AAGCAGAAGACGGCATACGAG | CTCGTATGCCGTCTTCTGCTT | 100.00% | gnl|uv|NGB00380.1:1-26 Illumina Small RNA 3' Adapter (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 1370 | 0.20% | ACGCGATGCCTTCACAAGTCT | AGACTTGTGAAGGCATCGCGT | 80.95% | gnl|uv|U59231.1:5103-7490 Cloning vector cLHYGpk |
| 1366 | 0.20% | CGCGATGCCTTCACAAGTCTC | GAGACTTGTGAAGGCATCGCG | 80.95% | gnl|uv|U59231.1:5103-7490 Cloning vector cLHYGpk |
| 1330 | 0.19% | TATGCCGTCTTCTGCTTGAAA | TTTCAAGCAGAAGACGGCATA | 95.24% | gnl|uv|JX069761.1:1-102 Cloning vector pFosill-1 |
| 1328 | 0.19% | CAAGCAGAAGACGGCATACGA | TCGTATGCCGTCTTCTGCTTG | 100.00% | gnl|uv|NGB00379.1:1-23 Illumina 3' RNA Adapter (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 1243 | 0.18% | ATGCCGTCTTCTGCTTGAAAA | TTTTCAAGCAGAAGACGGCAT | 90.48% | gnl|uv|JX069761.1:1-102 Cloning vector pFosill-1 |
| 1222 | 0.18% | ACCTGTCTCTTATACACATCT | AGATGTGTATAAGAGACAGGT | 100.00% | gnl|uv|JN835257.1:94-190 Cloning vector pIGWZMCS |
| 1186 | 0.17% | GACTTGTGAAGGCATCGCGTA | TACGCGATGCCTTCACAAGTC | 80.95% | gnl|uv|U59231.1:5103-7490 Cloning vector cLHYGpk |
| 1154 | 0.17% | ACTTGTGAAGGCATCGCGTAT | ATACGCGATGCCTTCACAAGT | 80.95% | gnl|uv|U59231.1:5103-7490 Cloning vector cLHYGpk |
| 1125 | 0.16% | CATACGCGATGCCTTCACAAG | CTTGTGAAGGCATCGCGTATG | 76.19% | gnl|uv|JQ437370.1:1-703 Nanoluc luciferase reporter vector pNL1.1[Nluc] |
| 1097 | 0.16% | CGGCATACGCGATGCCTTCAC | GTGAAGGCATCGCGTATGCCG | 76.19% | gnl|uv|X66730.1:1-2687-49 B.bronchiseptica plasmid pBBR1 genes for mobilization and replication |
| 1055 | 0.15% | GCATACGCGATGCCTTCACAA | TTGTGAAGGCATCGCGTATGC | 76.19% | gnl|uv|JQ437370.1:1-703 Nanoluc luciferase reporter vector pNL1.1[Nluc] |
| 1046 | 0.15% | GGCATACGCGATGCCTTCACA | TGTGAAGGCATCGCGTATGCC | 76.19% | gnl|uv|JQ437370.1:1-703 Nanoluc luciferase reporter vector pNL1.1[Nluc] |
| 1038 | 0.15% | GAAGGCATCGCGTATGCCGTC | GACGGCATACGCGATGCCTTC | 80.95% | gnl|uv|X66730.1:1-2687-49 B.bronchiseptica plasmid pBBR1 genes for mobilization and replication |
| 1022 | 0.15% | AGATGTGTATAAGAGACAGCT | AGCTGTCTCTTATACACATCT | 100.00% | gnl|uv|AB777652.1:4182-4357 Cloning vector pRMTn-Gm DNA |
| 960 | 0.14% | ACGGCATACGCGATGCCTTCA | TGAAGGCATCGCGTATGCCGT | 76.19% | gnl|uv|X66730.1:1-2687-49 B.bronchiseptica plasmid pBBR1 genes for mobilization and replication |
| 954 | 0.14% | AAGGCATCGCGTATGCCGTCT | AGACGGCATACGCGATGCCTT | 76.19% | gnl|uv|X66730.1:1-2687-49 B.bronchiseptica plasmid pBBR1 genes for mobilization and replication |
| 926 | 0.14% | CTTTTCAAGCAGAAGACGGCA | TGCCGTCTTCTGCTTGAAAAG | 85.71% | gnl|uv|JX069761.1:1-102 Cloning vector pFosill-1 |
| 811 | 0.12% | CCTTTTCAAGCAGAAGACGGC | GCCGTCTTCTGCTTGAAAAGG | 80.95% | gnl|uv|JX069761.1:1-102 Cloning vector pFosill-1 |
| 796 | 0.12% | AGATGTGTATAAGAGACAGGA | TCCTGTCTCTTATACACATCT | 95.24% | gnl|uv|JN835257.1:94-190 Cloning vector pIGWZMCS |
| 759 | 0.11% | AAGACGGCATACGCGATGCCT | AGGCATCGCGTATGCCGTCTT | 95.24% | gnl|uv|NGB00366.1:1-43 Illumina Multiplexing PCR Primer Index 3 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 759 | 0.11% | AACTGTCTCTTATACACATCT | AGATGTGTATAAGAGACAGTT | 95.24% | gnl|uv|NGB00401.1:1458-1518 pPDM-2 Cloning Vector |
| 752 | 0.11% | AGATGTGTATAAGAGACAGGC | GCCTGTCTCTTATACACATCT | 95.24% | gnl|uv|JN835257.1:94-190 Cloning vector pIGWZMCS |
| 745 | 0.11% | CCCTTTTCAAGCAGAAGACGG | CCGTCTTCTGCTTGAAAAGGG | 76.19% | gnl|uv|JX069761.1:1-102 Cloning vector pFosill-1 |
| 721 | 0.11% | CCCCTTTTCAAGCAGAAGACG | CGTCTTCTGCTTGAAAAGGGG | 71.43% | gnl|uv|JX069761.1:1-102 Cloning vector pFosill-1 |
| 719 | 0.11% | GAAGACGGCATACGCGATGCC | GGCATCGCGTATGCCGTCTTC | 95.24% | gnl|uv|NGB00366.1:1-43 Illumina Multiplexing PCR Primer Index 3 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
Read 2: Overrepresented sequences
A subsample of the sequences is analysed Sequences are cut into fragments of up to 31 bp. Fragments are stored and counted. When the maximum amount of unique fragments is reached, only fragments that are already stored are counted. The rest of the fragments is ignored. Fragments are stored in their canonical representation. That is either the sequence or the reverse complement, whichever has the lowest sort order. Both representations are shown in the table. See the documentation for a complete explanation. The sequence identity is calculated by .
The percentage shown is an estimate based on the number of occurences of the fragment in relation to the number of sampled sequences. Fragments are only counted once per sequence. Fragments that occur more than once in a sequence are counted as one.
| Total sequences in file | 5,477,353 |
| Sampled sequences | 684,670 |
| Sampling rate | 1 in 8 |
| Total fragments sampled | 5,476,939 |
| Stored unique fragments | 1,885,977 |
| Maximum unique fragments | 5,000,000 |
| Fragment size | 21 |
| count | percentage | canonical sequence | reverse complemented sequence | sequence identity | best match |
|---|---|---|---|---|---|
| 9949 | 1.45% | CTGTCTCTTATACACATCTGA | TCAGATGTGTATAAGAGACAG | 100.00% | gnl|uv|NGB00725.1:1-33 Illumina Nextera transposase sequence Read 1 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 9722 | 1.42% | GTCAGATGTGTATAAGAGACA | TGTCTCTTATACACATCTGAC | 100.00% | gnl|uv|NGB00725.1:1-33 Illumina Nextera transposase sequence Read 1 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 9443 | 1.38% | CGTCAGATGTGTATAAGAGAC | GTCTCTTATACACATCTGACG | 100.00% | gnl|uv|NGB00725.1:1-33 Illumina Nextera transposase sequence Read 1 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 9375 | 1.37% | GCGTCAGATGTGTATAAGAGA | TCTCTTATACACATCTGACGC | 100.00% | gnl|uv|NGB00725.1:1-33 Illumina Nextera transposase sequence Read 1 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 9372 | 1.37% | AGCGTCAGATGTGTATAAGAG | CTCTTATACACATCTGACGCT | 100.00% | gnl|uv|NGB00725.1:1-33 Illumina Nextera transposase sequence Read 1 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 9046 | 1.32% | CTTATACACATCTGACGCTGC | GCAGCGTCAGATGTGTATAAG | 100.00% | gnl|uv|NGB00725.1:1-33 Illumina Nextera transposase sequence Read 1 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 9004 | 1.32% | CAGCGTCAGATGTGTATAAGA | TCTTATACACATCTGACGCTG | 100.00% | gnl|uv|NGB00725.1:1-33 Illumina Nextera transposase sequence Read 1 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 8663 | 1.27% | GGCAGCGTCAGATGTGTATAA | TTATACACATCTGACGCTGCC | 100.00% | gnl|uv|NGB00725.1:1-33 Illumina Nextera transposase sequence Read 1 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 8481 | 1.24% | CGGCAGCGTCAGATGTGTATA | TATACACATCTGACGCTGCCG | 100.00% | gnl|uv|NGB00725.1:1-33 Illumina Nextera transposase sequence Read 1 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 8372 | 1.22% | ATACACATCTGACGCTGCCGA | TCGGCAGCGTCAGATGTGTAT | 100.00% | gnl|uv|NGB00725.1:1-33 Illumina Nextera transposase sequence Read 1 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 8108 | 1.18% | GTCGGCAGCGTCAGATGTGTA | TACACATCTGACGCTGCCGAC | 100.00% | gnl|uv|NGB00725.1:1-33 Illumina Nextera transposase sequence Read 1 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 7912 | 1.16% | ACACATCTGACGCTGCCGACG | CGTCGGCAGCGTCAGATGTGT | 100.00% | gnl|uv|NGB00725.1:1-33 Illumina Nextera transposase sequence Read 1 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 7734 | 1.13% | CACATCTGACGCTGCCGACGA | TCGTCGGCAGCGTCAGATGTG | 100.00% | gnl|uv|NGB00725.1:1-33 Illumina Nextera transposase sequence Read 1 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 7512 | 1.10% | ACATCTGACGCTGCCGACGAT | ATCGTCGGCAGCGTCAGATGT | 95.24% | gnl|uv|NGB00725.1:1-33 Illumina Nextera transposase sequence Read 1 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 7496 | 1.09% | CATCTGACGCTGCCGACGATC | GATCGTCGGCAGCGTCAGATG | 90.48% | gnl|uv|NGB00725.1:1-33 Illumina Nextera transposase sequence Read 1 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 7352 | 1.07% | ATCTGACGCTGCCGACGATCG | CGATCGTCGGCAGCGTCAGAT | 85.71% | gnl|uv|NGB00725.1:1-33 Illumina Nextera transposase sequence Read 1 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 7156 | 1.05% | ACGATCGTCGGCAGCGTCAGA | TCTGACGCTGCCGACGATCGT | 80.95% | gnl|uv|NGB00725.1:1-33 Illumina Nextera transposase sequence Read 1 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 7074 | 1.03% | TGACGATCGTCGGCAGCGTCA | TGACGCTGCCGACGATCGTCA | 80.95% | gnl|uv|NGB00730.1:1-51 Illumina Nextera PCR primer i5 index N504 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 6988 | 1.02% | CTGACGCTGCCGACGATCGTC | GACGATCGTCGGCAGCGTCAG | 80.95% | gnl|uv|NGB00730.1:1-51 Illumina Nextera PCR primer i5 index N504 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 6845 | 1.00% | CTGACGATCGTCGGCAGCGTC | GACGCTGCCGACGATCGTCAG | 85.71% | gnl|uv|NGB00732.1:1-51 Illumina Nextera PCR primer i5 index N506 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 6683 | 0.98% | ACGCTGCCGACGATCGTCAGT | ACTGACGATCGTCGGCAGCGT | 85.71% | gnl|uv|NGB00732.1:1-51 Illumina Nextera PCR primer i5 index N506 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 6377 | 0.93% | CGCTGCCGACGATCGTCAGTA | TACTGACGATCGTCGGCAGCG | 80.95% | gnl|uv|NGB00732.1:1-51 Illumina Nextera PCR primer i5 index N506 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 6261 | 0.91% | GCTGCCGACGATCGTCAGTAC | GTACTGACGATCGTCGGCAGC | 71.43% | gnl|uv|NGB00730.1:1-51 Illumina Nextera PCR primer i5 index N504 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 6019 | 0.88% | CGTACTGACGATCGTCGGCAG | CTGCCGACGATCGTCAGTACG | 80.95% | gnl|uv|KY031735.1:1-4680 Cloning vector pABC4 |
| 5961 | 0.87% | ACGTACTGACGATCGTCGGCA | TGCCGACGATCGTCAGTACGT | 76.19% | gnl|uv|JN874480.1:31317-31418 Cloning vector pHUGE-Red |
| 5891 | 0.86% | CACGTACTGACGATCGTCGGC | GCCGACGATCGTCAGTACGTG | 80.95% | gnl|uv|NGB01096.1:1-66 Rubicon Genomics ThruPLEX DNA-seq dual-index D508 |
| 5591 | 0.82% | CGACGATCGTCAGTACGTGTA | TACACGTACTGACGATCGTCG | 76.19% | gnl|uv|NGB00754.1:1-70 Illumina TruSeq DNA HT and RNA HT i5 index D508 adapter (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 5579 | 0.81% | ACACGTACTGACGATCGTCGG | CCGACGATCGTCAGTACGTGT | 80.95% | gnl|uv|JN874480.1:31317-31418 Cloning vector pHUGE-Red |
| 5452 | 0.80% | CTACACGTACTGACGATCGTC | GACGATCGTCAGTACGTGTAG | 80.95% | gnl|uv|NGB00754.1:1-70 Illumina TruSeq DNA HT and RNA HT i5 index D508 adapter (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 5203 | 0.76% | ACGATCGTCAGTACGTGTAGA | TCTACACGTACTGACGATCGT | 80.95% | gnl|uv|NGB00754.1:1-70 Illumina TruSeq DNA HT and RNA HT i5 index D508 adapter (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 5183 | 0.76% | ATCTACACGTACTGACGATCG | CGATCGTCAGTACGTGTAGAT | 85.71% | gnl|uv|NGB00754.1:1-70 Illumina TruSeq DNA HT and RNA HT i5 index D508 adapter (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 4850 | 0.71% | GATCGTCAGTACGTGTAGATC | GATCTACACGTACTGACGATC | 90.48% | gnl|uv|NGB00754.1:1-70 Illumina TruSeq DNA HT and RNA HT i5 index D508 adapter (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 4691 | 0.69% | AGATCTACACGTACTGACGAT | ATCGTCAGTACGTGTAGATCT | 90.48% | gnl|uv|NGB00754.1:1-70 Illumina TruSeq DNA HT and RNA HT i5 index D508 adapter (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 4559 | 0.67% | GAGATCTACACGTACTGACGA | TCGTCAGTACGTGTAGATCTC | 95.24% | gnl|uv|NGB00754.1:1-70 Illumina TruSeq DNA HT and RNA HT i5 index D508 adapter (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 4378 | 0.64% | CGAGATCTACACGTACTGACG | CGTCAGTACGTGTAGATCTCG | 95.24% | gnl|uv|NGB00754.1:1-70 Illumina TruSeq DNA HT and RNA HT i5 index D508 adapter (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 4323 | 0.63% | CCGAGATCTACACGTACTGAC | GTCAGTACGTGTAGATCTCGG | 100.00% | gnl|uv|NGB00754.1:1-70 Illumina TruSeq DNA HT and RNA HT i5 index D508 adapter (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 4274 | 0.62% | ACCGAGATCTACACGTACTGA | TCAGTACGTGTAGATCTCGGT | 100.00% | gnl|uv|NGB00754.1:1-70 Illumina TruSeq DNA HT and RNA HT i5 index D508 adapter (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 4075 | 0.60% | CACCGAGATCTACACGTACTG | CAGTACGTGTAGATCTCGGTG | 100.00% | gnl|uv|NGB00754.1:1-70 Illumina TruSeq DNA HT and RNA HT i5 index D508 adapter (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 4010 | 0.59% | AGTACGTGTAGATCTCGGTGG | CCACCGAGATCTACACGTACT | 100.00% | gnl|uv|NGB00754.1:1-70 Illumina TruSeq DNA HT and RNA HT i5 index D508 adapter (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 3968 | 0.58% | ACCACCGAGATCTACACGTAC | GTACGTGTAGATCTCGGTGGT | 100.00% | gnl|uv|NGB00754.1:1-70 Illumina TruSeq DNA HT and RNA HT i5 index D508 adapter (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 3830 | 0.56% | GACCACCGAGATCTACACGTA | TACGTGTAGATCTCGGTGGTC | 100.00% | gnl|uv|NGB00731.1:1-51 Illumina Nextera PCR primer i5 index N505 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 3817 | 0.56% | CCCCCCCCCCCCCCCCCCCCC | GGGGGGGGGGGGGGGGGGGGG | 100.00% | Poly-G. Illumina dark cycle pattern. |
| 3697 | 0.54% | ACGTGTAGATCTCGGTGGTCG | CGACCACCGAGATCTACACGT | 100.00% | gnl|uv|NGB00793.1:1-50 Illumina TruSeq Small RNA Sample Prep Kit RNA PCR Primer (RP1) (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 3500 | 0.51% | CGTGTAGATCTCGGTGGTCGC | GCGACCACCGAGATCTACACG | 100.00% | gnl|uv|NGB00844.1:1-48 Epicentre Biotechnologies Nextera DNA Sample Prep Kit Adaptor (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 3494 | 0.51% | CAGATGTGTATAAGAGACAGG | CCTGTCTCTTATACACATCTG | 95.24% | gnl|uv|NGB00725.1:1-33 Illumina Nextera transposase sequence Read 1 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 3363 | 0.49% | GGCGACCACCGAGATCTACAC | GTGTAGATCTCGGTGGTCGCC | 100.00% | gnl|uv|NGB00844.1:1-48 Epicentre Biotechnologies Nextera DNA Sample Prep Kit Adaptor (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 3265 | 0.48% | CGGCGACCACCGAGATCTACA | TGTAGATCTCGGTGGTCGCCG | 100.00% | gnl|uv|NGB00844.1:1-48 Epicentre Biotechnologies Nextera DNA Sample Prep Kit Adaptor (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 3048 | 0.45% | CAGATGTGTATAAGAGACAGA | TCTGTCTCTTATACACATCTG | 95.24% | gnl|uv|NGB00725.1:1-33 Illumina Nextera transposase sequence Read 1 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 2951 | 0.43% | ACGGCGACCACCGAGATCTAC | GTAGATCTCGGTGGTCGCCGT | 100.00% | gnl|uv|NGB00844.1:1-48 Epicentre Biotechnologies Nextera DNA Sample Prep Kit Adaptor (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 2819 | 0.41% | TACGGCGACCACCGAGATCTA | TAGATCTCGGTGGTCGCCGTA | 100.00% | gnl|uv|NGB00844.1:1-48 Epicentre Biotechnologies Nextera DNA Sample Prep Kit Adaptor (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 2485 | 0.36% | GATACGGCGACCACCGAGATC | GATCTCGGTGGTCGCCGTATC | 100.00% | gnl|uv|NGB00844.1:1-48 Epicentre Biotechnologies Nextera DNA Sample Prep Kit Adaptor (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 2449 | 0.36% | AGATCTCGGTGGTCGCCGTAT | ATACGGCGACCACCGAGATCT | 100.00% | gnl|uv|NGB00844.1:1-48 Epicentre Biotechnologies Nextera DNA Sample Prep Kit Adaptor (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 2367 | 0.35% | ATCTCGGTGGTCGCCGTATCA | TGATACGGCGACCACCGAGAT | 100.00% | gnl|uv|NGB00844.1:1-48 Epicentre Biotechnologies Nextera DNA Sample Prep Kit Adaptor (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 2216 | 0.32% | ATGATACGGCGACCACCGAGA | TCTCGGTGGTCGCCGTATCAT | 100.00% | gnl|uv|NGB00844.1:1-48 Epicentre Biotechnologies Nextera DNA Sample Prep Kit Adaptor (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 2185 | 0.32% | AATGATACGGCGACCACCGAG | CTCGGTGGTCGCCGTATCATT | 100.00% | gnl|uv|NGB00844.1:1-48 Epicentre Biotechnologies Nextera DNA Sample Prep Kit Adaptor (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 2089 | 0.31% | CAGATGTGTATAAGAGACAGC | GCTGTCTCTTATACACATCTG | 100.00% | gnl|uv|AB777652.1:5733-7306-49 Cloning vector pRMTn-Gm DNA |
| 2014 | 0.29% | TAATGATACGGCGACCACCGA | TCGGTGGTCGCCGTATCATTA | 100.00% | gnl|uv|JX069762.1:9774-9902-49 Cloning vector pFosill-2 |
| 1832 | 0.27% | CGGTGGTCGCCGTATCATTAA | TTAATGATACGGCGACCACCG | 100.00% | gnl|uv|JX069762.1:9774-9902-49 Cloning vector pFosill-2 |
| 1765 | 0.26% | GGTGGTCGCCGTATCATTAAA | TTTAATGATACGGCGACCACC | 95.24% | gnl|uv|JX069762.1:9774-9902-49 Cloning vector pFosill-2 |
| 1689 | 0.25% | GTGGTCGCCGTATCATTAAAA | TTTTAATGATACGGCGACCAC | 90.48% | gnl|uv|JX069762.1:9774-9902-49 Cloning vector pFosill-2 |
| 1550 | 0.23% | AGATGTGTATAAGAGACAGAT | ATCTGTCTCTTATACACATCT | 100.00% | gnl|uv|NGB00399.1:2658-2791 pCDC-1K Cloning Vector |
| 1522 | 0.22% | ACTGTCTCTTATACACATCTG | CAGATGTGTATAAGAGACAGT | 95.24% | gnl|uv|NGB00725.1:1-33 Illumina Nextera transposase sequence Read 1 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.) |
| 1424 | 0.21% | TGGTCGCCGTATCATTAAAAA | TTTTTAATGATACGGCGACCA | 85.71% | gnl|uv|JX069762.1:9774-9902-49 Cloning vector pFosill-2 |
| 1295 | 0.19% | ACCTGTCTCTTATACACATCT | AGATGTGTATAAGAGACAGGT | 100.00% | gnl|uv|JN835257.1:94-190 Cloning vector pIGWZMCS |
| 1080 | 0.16% | AGATGTGTATAAGAGACAGCT | AGCTGTCTCTTATACACATCT | 100.00% | gnl|uv|AB777652.1:4182-4357 Cloning vector pRMTn-Gm DNA |
| 855 | 0.12% | CTTTTTAATGATACGGCGACC | GGTCGCCGTATCATTAAAAAG | 80.95% | gnl|uv|JX069762.1:9774-9902-49 Cloning vector pFosill-2 |
| 844 | 0.12% | AGATGTGTATAAGAGACAGGA | TCCTGTCTCTTATACACATCT | 95.24% | gnl|uv|JN835257.1:94-190 Cloning vector pIGWZMCS |
| 773 | 0.11% | AGATGTGTATAAGAGACAGGC | GCCTGTCTCTTATACACATCT | 95.24% | gnl|uv|JN835257.1:94-190 Cloning vector pIGWZMCS |
| 722 | 0.11% | AACTGTCTCTTATACACATCT | AGATGTGTATAAGAGACAGTT | 95.24% | gnl|uv|NGB00401.1:1458-1518 pPDM-2 Cloning Vector |
| 699 | 0.10% | CCCCTTTTTAATGATACGGCG | CGCCGTATCATTAAAAAGGGG | 66.67% | gnl|uv|JX069762.1:9774-9902-49 Cloning vector pFosill-2 |
| 693 | 0.10% | CCCTTTTTAATGATACGGCGA | TCGCCGTATCATTAAAAAGGG | 71.43% | gnl|uv|JX069762.1:9774-9902-49 Cloning vector pFosill-2 |