Sequali report

Table of contents

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Metadata

Filename21C125_R1.fastq.gz
Filesize0.45 GiB
Filename read 2 21C125_R2.fastq.gz
Filesize read 2 0.46 GiB
Sequali version0.10.1.dev10+g545d2be
Report generated on2024-06-10 06:33:03+0200

Read 1: Summary

Mean length 151.00
Length range (min-max) 151 151
Total reads 5,477,353
Q20 reads 5,214,420 95.20%
Total bases 827,080,303
Total GC bases 273,860,586 33.11%
Q20 bases 798,680,482 96.57%

Read 2: Summary

Mean length 151.00
Length range (min-max) 151 151
Total reads 5,477,353
Q20 reads 5,243,046 95.72%
Total bases 827,080,303
Total GC bases 273,545,195 33.07%
Q20 bases 797,522,582 96.43%

Read 1: Sequence length distribution

Sequence length distribution001000000100000020000002000000300000030000004000000400000050000005000000051015202530354045505560657075808590951001051101151201251301351401451500023.146153846153844568.846153846153801029.684615384615384568.846153846153812036.22307692307692568.846153846153823042.76153846153845568.846153846153834049.3568.846153846153845055.83846153846152568.846153846153856062.37692307692306568.846153846153867068.91538461538461568.846153846153878075.45384615384614568.846153846153889081.99230769230768568.8461538461538910088.53076923076922568.84615384615381011095.06923076923077568.846153846153811120101.6076923076923568.846153846153812130108.14615384615385568.846153846153813140114.68461538461537568.846153846153814150121.22307692307692568.846153846153815160127.76153846153844568.846153846153816170134.29999999999998568.846153846153817180140.8384615384615568.846153846153818190147.37692307692305568.846153846153819200153.9153846153846568.846153846153820210160.45384615384614568.846153846153821220166.9923076923077568.846153846153822230173.5307692307692568.846153846153823240180.06923076923073568.846153846153824250186.6076923076923568.846153846153825260193.14615384615382568.846153846153826270199.68461538461534568.846153846153827280206.2230769230769568.846153846153828290212.76153846153844568.846153846153829300219.29999999999995568.846153846153830310225.8384615384615568.846153846153831320232.37692307692305568.846153846153832330238.9153846153846568.846153846153833340245.45384615384611568.846153846153834350251.99230769230766568.846153846153835360258.5307692307692568.846153846153836370265.0692307692307568.846153846153837380271.60769230769233568.846153846153838390278.1461538461539568.846153846153839400284.68461538461537568.846153846153840410291.2230769230769568.846153846153841420297.76153846153846568.846153846153842430304.3568.846153846153843440310.83846153846156568.846153846153844450317.37692307692305568.846153846153845460323.9153846153846568.846153846153846470330.45384615384614568.846153846153847480336.99230769230763568.846153846153848490343.53076923076924568.846153846153849500350.0692307692308568.846153846153850510356.6076923076923568.846153846153851520363.1461538461538568.846153846153852530369.6846153846153568.846153846153853540376.22307692307686568.846153846153854550382.76153846153846568.846153846153855560389.29999999999995568.846153846153856570395.8384615384615568.846153846153857580402.37692307692305568.846153846153858590408.91538461538454568.846153846153859600415.4538461538461568.846153846153860610421.9923076923077568.846153846153861620428.53076923076924568.846153846153862630435.0692307692308568.846153846153863640441.6076923076923568.846153846153864650448.1461538461538568.846153846153865660454.68461538461537568.846153846153866670461.22307692307686568.846153846153867680467.76153846153846568.846153846153868690474.3568.846153846153869700480.8384615384615568.846153846153870710487.37692307692305568.846153846153871720493.91538461538454568.846153846153872730500.45384615384614568.846153846153873740506.99230769230763568.846153846153874750513.5307692307691568.846153846153875760520.0692307692307568.846153846153876770526.6076923076922568.846153846153877780533.1461538461538568.846153846153878790539.6846153846153568.846153846153879800546.2230769230767568.846153846153880810552.7615384615384568.846153846153881820559.2999999999998568.846153846153882830565.8384615384614568.846153846153883840572.376923076923568.846153846153884850578.9153846153846568.846153846153885860585.453846153846568.846153846153886870591.9923076923076568.846153846153887880598.5307692307691568.846153846153888890605.0692307692307568.846153846153889900611.6076923076921568.846153846153890910618.1461538461538568.846153846153891920624.6846153846152568.846153846153892930631.2230769230767568.846153846153893940637.7615384615383568.846153846153894950644.2999999999998568.846153846153895960650.8384615384614568.846153846153896970657.376923076923568.846153846153897980663.9153846153846568.846153846153898990670.453846153846568.8461538461538991000676.9923076923077568.84615384615381001010683.5307692307691568.84615384615381011020690.0692307692307568.84615384615381021030696.6076923076922568.84615384615381031040703.1461538461538568.84615384615381041050709.6846153846152568.84615384615381051060716.2230769230767568.84615384615381061070722.7615384615383568.84615384615381071080729.2999999999998568.84615384615381081090735.8384615384614568.84615384615381091100742.376923076923568.84615384615381101110748.9153846153845568.84615384615381111120755.453846153846568.84615384615381121130761.9923076923076568.84615384615381131140768.5307692307691568.84615384615381141150775.0692307692306568.84615384615381151160781.6076923076922568.84615384615381161170788.1461538461537568.84615384615381171180794.6846153846152568.84615384615381181190801.2230769230767568.84615384615381191200807.7615384615383568.84615384615381201210814.2999999999998568.84615384615381211220820.8384615384615568.84615384615381221230827.376923076923568.84615384615381231240833.9153846153845568.84615384615381241250840.453846153846568.84615384615381251260846.9923076923076568.84615384615381261270853.5307692307691568.84615384615381271280860.0692307692306568.84615384615381281290866.6076923076922568.84615384615381291300873.1461538461537568.84615384615381301310879.6846153846152568.84615384615381311320886.2230769230767568.84615384615381321330892.7615384615383568.84615384615381331340899.2999999999997568.84615384615381341350905.8384615384615568.84615384615381351360912.3769230769229568.84615384615381361370918.9153846153845568.84615384615381371380925.453846153846568.84615384615381381390931.9923076923076568.84615384615381391400938.530769230769568.84615384615381401410945.0692307692307568.84615384615381411420951.6076923076921568.84615384615381421430958.1461538461537568.84615384615381431440964.6846153846152568.84615384615381441450971.2230769230767568.84615384615381451460977.7615384615383568.84615384615381461470984.2999999999998568.84615384615381471480990.8384615384614568.84615384615381481490997.3769230769228568.846153846153814915001003.9153846153844568.846153846153815015154773531010.453846153846289.99999999999994151Sequence length distributionsequence lengthnumber of readsLength

Read 2: Sequence length distribution

Sequence length distribution001000000100000020000002000000300000030000004000000400000050000005000000051015202530354045505560657075808590951001051101151201251301351401451500023.146153846153844568.846153846153801029.684615384615384568.846153846153812036.22307692307692568.846153846153823042.76153846153845568.846153846153834049.3568.846153846153845055.83846153846152568.846153846153856062.37692307692306568.846153846153867068.91538461538461568.846153846153878075.45384615384614568.846153846153889081.99230769230768568.8461538461538910088.53076923076922568.84615384615381011095.06923076923077568.846153846153811120101.6076923076923568.846153846153812130108.14615384615385568.846153846153813140114.68461538461537568.846153846153814150121.22307692307692568.846153846153815160127.76153846153844568.846153846153816170134.29999999999998568.846153846153817180140.8384615384615568.846153846153818190147.37692307692305568.846153846153819200153.9153846153846568.846153846153820210160.45384615384614568.846153846153821220166.9923076923077568.846153846153822230173.5307692307692568.846153846153823240180.06923076923073568.846153846153824250186.6076923076923568.846153846153825260193.14615384615382568.846153846153826270199.68461538461534568.846153846153827280206.2230769230769568.846153846153828290212.76153846153844568.846153846153829300219.29999999999995568.846153846153830310225.8384615384615568.846153846153831320232.37692307692305568.846153846153832330238.9153846153846568.846153846153833340245.45384615384611568.846153846153834350251.99230769230766568.846153846153835360258.5307692307692568.846153846153836370265.0692307692307568.846153846153837380271.60769230769233568.846153846153838390278.1461538461539568.846153846153839400284.68461538461537568.846153846153840410291.2230769230769568.846153846153841420297.76153846153846568.846153846153842430304.3568.846153846153843440310.83846153846156568.846153846153844450317.37692307692305568.846153846153845460323.9153846153846568.846153846153846470330.45384615384614568.846153846153847480336.99230769230763568.846153846153848490343.53076923076924568.846153846153849500350.0692307692308568.846153846153850510356.6076923076923568.846153846153851520363.1461538461538568.846153846153852530369.6846153846153568.846153846153853540376.22307692307686568.846153846153854550382.76153846153846568.846153846153855560389.29999999999995568.846153846153856570395.8384615384615568.846153846153857580402.37692307692305568.846153846153858590408.91538461538454568.846153846153859600415.4538461538461568.846153846153860610421.9923076923077568.846153846153861620428.53076923076924568.846153846153862630435.0692307692308568.846153846153863640441.6076923076923568.846153846153864650448.1461538461538568.846153846153865660454.68461538461537568.846153846153866670461.22307692307686568.846153846153867680467.76153846153846568.846153846153868690474.3568.846153846153869700480.8384615384615568.846153846153870710487.37692307692305568.846153846153871720493.91538461538454568.846153846153872730500.45384615384614568.846153846153873740506.99230769230763568.846153846153874750513.5307692307691568.846153846153875760520.0692307692307568.846153846153876770526.6076923076922568.846153846153877780533.1461538461538568.846153846153878790539.6846153846153568.846153846153879800546.2230769230767568.846153846153880810552.7615384615384568.846153846153881820559.2999999999998568.846153846153882830565.8384615384614568.846153846153883840572.376923076923568.846153846153884850578.9153846153846568.846153846153885860585.453846153846568.846153846153886870591.9923076923076568.846153846153887880598.5307692307691568.846153846153888890605.0692307692307568.846153846153889900611.6076923076921568.846153846153890910618.1461538461538568.846153846153891920624.6846153846152568.846153846153892930631.2230769230767568.846153846153893940637.7615384615383568.846153846153894950644.2999999999998568.846153846153895960650.8384615384614568.846153846153896970657.376923076923568.846153846153897980663.9153846153846568.846153846153898990670.453846153846568.8461538461538991000676.9923076923077568.84615384615381001010683.5307692307691568.84615384615381011020690.0692307692307568.84615384615381021030696.6076923076922568.84615384615381031040703.1461538461538568.84615384615381041050709.6846153846152568.84615384615381051060716.2230769230767568.84615384615381061070722.7615384615383568.84615384615381071080729.2999999999998568.84615384615381081090735.8384615384614568.84615384615381091100742.376923076923568.84615384615381101110748.9153846153845568.84615384615381111120755.453846153846568.84615384615381121130761.9923076923076568.84615384615381131140768.5307692307691568.84615384615381141150775.0692307692306568.84615384615381151160781.6076923076922568.84615384615381161170788.1461538461537568.84615384615381171180794.6846153846152568.84615384615381181190801.2230769230767568.84615384615381191200807.7615384615383568.84615384615381201210814.2999999999998568.84615384615381211220820.8384615384615568.84615384615381221230827.376923076923568.84615384615381231240833.9153846153845568.84615384615381241250840.453846153846568.84615384615381251260846.9923076923076568.84615384615381261270853.5307692307691568.84615384615381271280860.0692307692306568.84615384615381281290866.6076923076922568.84615384615381291300873.1461538461537568.84615384615381301310879.6846153846152568.84615384615381311320886.2230769230767568.84615384615381321330892.7615384615383568.84615384615381331340899.2999999999997568.84615384615381341350905.8384615384615568.84615384615381351360912.3769230769229568.84615384615381361370918.9153846153845568.84615384615381371380925.453846153846568.84615384615381381390931.9923076923076568.84615384615381391400938.530769230769568.84615384615381401410945.0692307692307568.84615384615381411420951.6076923076921568.84615384615381421430958.1461538461537568.84615384615381431440964.6846153846152568.84615384615381441450971.2230769230767568.84615384615381451460977.7615384615383568.84615384615381461470984.2999999999998568.84615384615381471480990.8384615384614568.84615384615381481490997.3769230769228568.846153846153814915001003.9153846153844568.846153846153815015154773531010.453846153846289.99999999999994151Sequence length distributionsequence lengthnumber of readsLength

Read 1: Per position quality score distribution

Per base quality distribution000.10.10.20.20.30.30.40.40.50.50.60.60.70.70.80.80.90.9111611162126313641465156616671768186919610110611111612112613113614114615119.165010909e-0524.136118186449313568.8205975657331120.000927455287330.992969943963324568.5875365064392230.000203565481437.849821701477325568.789390394621934044.70667345899134568.846153846153845051.56352521650534568.846153846153856058.42037697401936568.846153846153867065.27722873153336568.846153846153878072.13408048904736568.846153846153889078.99093224656137568.8461538461538910085.8477840040754568.84615384615381011092.7046357615894568.84615384615381112099.5614875191034568.846153846153812131.825699384e-06106.4183392766174568.845644756902413140113.27519103413142568.846153846153814150120.13204279164543568.846153846153815165.659668092e-06126.98889454915944568.844575669474516172.555979138e-06133.84574630667345568.845441121201917182.081297298e-05140.70259806418747568.840350228687818190147.55944982170143568.846153846153819200154.41630157921546568.846153846153820210161.27315333672948568.846153846153821220168.13000509424344568.846153846153822230174.9868568517575568.846153846153823240181.8437086092715568.846153846153824250188.7005603667855568.846153846153825260195.55741212429953568.846153846153826270202.4142638818135568.846153846153827280209.27111563932752568.846153846153828290216.1279673968415568.846153846153829300222.98481915435556568.846153846153830310229.84167091186956568.846153846153831320236.69852266938355568.846153846153832330243.55537442689757568.846153846153833340250.4122261844116568.846153846153834350257.2690779419256568.846153846153835360264.1259296994396568.846153846153836370270.98278145695366568.846153846153837387.302797537e-07277.8396332144676568.845950210453338390284.6964849719817568.846153846153839405.111958276e-06291.55333672949564568.844728396249840419.128496922e-07298.4101884870097568.845899301528141420305.26704024452374568.846153846153842430312.1238920020377568.846153846153843447.302797537e-07318.98074375955173568.845950210453344450325.8375955170657568.846153846153845463.651398769e-07332.6944472745798568.846052028303646470339.5512990320937568.846153846153847483.833968707e-06346.40815078960776568.845084758725948490353.2650025471218568.846153846153849500360.12185430463575568.846153846153850512.537722144e-05366.9787060621498568.839077505559351520.0005488052349373.8355578196638568.693121617180952531.259732575e-05380.6924095771778568.842641130319253540387.5492613346918568.846153846153854552.555979138e-06394.4061130922058568.845441121201955561.825699384e-07401.2629648497198568.846102937228756570408.1198166072338568.846153846153857582.3734092e-06414.97666836474787568.845492030126958590421.83352012226186568.846153846153859600428.69037187977585568.846153846153860614.929388338e-06435.54722363728985568.84477930517561622.738549077e-06442.4040753948039568.845390212276762637.302797537e-06449.2609271523179568.844117489148263645.477098153e-07456.1177789098319568.846001119378364651.022391655e-05462.97463066734593568.84330294634665661.460559507e-06469.83148242485987568.845746574752666672.555979138e-06476.6883341823739568.845441121201967680483.5451859398879568.846153846153868694.053052633e-05490.40203769740197568.834852064772669701.825699384e-07497.2588894549159568.846102937228770710504.11574121242995568.846153846153871720510.97259296994395568.846153846153872730517.8294447274579568.846153846153873744.381678522e-06524.686296484972568.844932031950574750531.5431482424859568.846153846153875760.0002369757801538.4568.780074061321276770545.256851757514568.846153846153877781.825699384e-07552.1137035150281568.846102937228778798.945926983e-06558.970555272542568.84365930882279800565.8274070300561568.846153846153880813.103688953e-06572.68425878757568.845288394426581822.026526317e-05579.541110545084568.840502955463282830586.3979623025981568.846153846153883843.103688953e-06593.254814060112568.845288394426584850600.1116658176261568.846153846153885861.825699384e-07606.9685175751401568.846102937228786870613.8253693326541568.846153846153887882.574236132e-05620.682221090168568.83897568770988890627.5390728476822568.846153846153889901.131933618e-05634.3959246051961568.842997492795190910641.2527763627102568.846153846153891925.111958276e-06648.1096281202241568.844728396249892930654.9664798777382568.846153846153893941.515330489e-05661.8233316352522568.841928405367194952.921119015e-06668.680183392766568.845339303351695961.314503557e-05675.5370351502802568.842488403543796970682.3938869077942568.846153846153897983.651398769e-07689.2507386653081568.846052028303698991.277989569e-05696.1075904228222568.842590221394991005.294528215e-06702.9644421803363568.84467748732481001010709.8212939378502568.84615384615381011025.477098153e-07716.6781456953642568.84600111937831021030723.5349974528783568.84615384615381031041.168447606e-05730.3918492103923568.84289567494491041051.095419631e-05737.2487009679062568.84309931064541051063.103688953e-06744.1055527254204568.84528839442651061070750.9624044829343568.8461538461538107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base quality distributionpositionfraction0-34-78-1112-1516-1920-2324-2728-3132-3536-3940-43>=44

Read 2: Per position quality score distribution

Per base quality distribution000.10.10.20.20.30.30.40.40.50.50.60.60.70.70.80.80.90.9111611162126313641465156616671768186919610110611111612112613113614114615111.588358464e-0524.136118186449313568.8417247696666123.286258892e-0630.992969943963324568.8452374855012235.477098153e-0737.849821701477325568.846001119378334044.70667345899134568.8461538461538456.207377907e-0651.56352521650534568.8444229426991562.190839261e-0658.42037697401936568.8455429390522672.501208157e-0565.27722873153336568.8391793234096783.103688953e-0672.13408048904736568.8452883944265891.42404552e-0578.99093224656137568.84218294999289107.667937414e-0685.8477840040754568.844015671297910113.048917972e-0592.7046357615894568.837652055655311123.103688953e-0699.5614875191034568.845288394426512131.168447606e-05106.4183392766174568.842895674944913141.825699384e-07113.27519103413142568.846102937228714152.099554292e-05120.13204279164543568.840299319762615169.128496922e-07126.98889454915944568.845899301528116171.825699384e-05133.84574630667345568.841062953639717180140.70259806418747568.846153846153818195.659668092e-06147.55944982170143568.844575669474519200154.41630157921546568.846153846153820211.442302514e-05161.27315333672948568.842132041067821229.128496922e-07168.13000509424344568.845899301528122232.464694169e-05174.9868568517575568.839281141259823242.190839261e-06181.8437086092715568.845542939052224251.478816501e-05188.7005603667855568.842030223217425261.204961594e-05195.55741212429953568.842793857094526276.389947845e-06202.4142638818135568.844372033773927281.095419631e-06209.27111563932752568.84584839260328297.850507353e-06216.1279673968415568.843964762372729300222.98481915435556568.846153846153830311.058905643e-05229.84167091186956568.843201128495631323.286258892e-06236.69852266938355568.845237485501232332.921119015e-06243.55537442689757568.845339303351633341.460559507e-06250.4122261844116568.845746574752634355.84223803e-06257.2690779419256568.844524760549335362.775063064e-05264.1259296994396568.838415689532436370270.98278145695366568.846153846153837387.302797537e-07277.8396332144676568.845950210453338391.643129446e-06284.6964849719817568.845695665827639401.42404552e-05291.55333672949564568.842182949992840410298.4101884870097568.846153846153841423.286258892e-06305.26704024452374568.845237485501242435.477098153e-07312.1238920020377568.846001119378343443.651398769e-07318.98074375955173568.846052028303644451.606615458e-05325.8375955170657568.841673860741445461.405788526e-05332.6944472745798568.84223385891846475.477098153e-07339.5512990320937568.846001119378347483.286258892e-06346.40815078960776568.845237485501248491.643129446e-06353.2650025471218568.845695665827649501.588358464e-05360.12185430463575568.841724769666650511.843956378e-05366.9787060621498568.841012044714651522.263867237e-05373.8355578196638568.839841139436452532.738549077e-06380.6924095771778568.845390212276753548.033077291e-06387.5492613346918568.843913853447754550394.4061130922058568.846153846153855562.318638218e-05401.2629648497198568.83968841266156573.651398769e-07408.1198166072338568.846052028303657585.111958276e-06414.97666836474787568.844728396249858595.111958276e-06421.83352012226186568.844728396249859601.643129446e-06428.69037187977585568.845695665827660613.833968707e-06435.54722363728985568.845084758725961627.302797537e-07442.4040753948039568.845950210453362631.825699384e-05449.2609271523179568.841062953639763642.008269323e-06456.1177789098319568.845593847977364659.676206737e-06462.97463066734593568.843455673121365663.46882883e-06469.83148242485987568.845186576576266672.61075012e-05476.6883341823739568.838873869858767680483.5451859398879568.846153846153868697.485367476e-06490.40203769740197568.844066580223169700497.2588894549159568.846153846153870718.945926983e-06504.11574121242995568.84365930882271723.286258892e-06510.97259296994395568.845237485501272736.572517784e-06517.8294447274579568.844321124848873745.111958276e-06524.686296484972568.844728396249874757.667937414e-06531.5431482424859568.844015671297975769.858776676e-06538.4568.843404764196376779.128496922e-07545.256851757514568.845899301528177781.990012329e-05552.1137035150281568.840604773313578793.194973923e-05558.970555272542568.837244784254279809.676206737e-06565.8274070300561568.843455673121380819.31106686e-06572.68425878757568.843557490971681820579.541110545084568.846153846153882831.332760551e-05586.3979623025981568.842437494618583841.825699384e-07593.254814060112568.846102937228784851.460559507e-05600.1116658176261568.842081132142585861.825699384e-06606.9685175751401568.845644756902486871.825699384e-06613.8253693326541568.845644756902487884.746818399e-06620.682221090168568.844830214100288892.555979138e-06627.5390728476822568.845441121201989903.651398769e-07634.3959246051961568.846052028303690911.825699384e-07641.2527763627102568.846102937228791920648.1096281202241568.846153846153892930654.9664798777382568.846153846153893941.825699384e-07661.8233316352522568.846102937228794951.077162637e-05668.680183392766568.843150219570695967.6679374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base quality distributionpositionfraction0-34-78-1112-1516-1920-2324-2728-3132-3536-3940-43>=44

Read 1: Per position quality percentiles

Shows the mean for all bases and the means of the lowest and highest percentiles to indicate the spread. Since the graph is based on the sampled categories, rather than exact phreds, it is an approximation.

Per position quality percentiles001010202030304040505016111621263136414651566166717681869196101106111116121126131136141146151Per position quality percentilespositionphred score125.3210406920.13076923076923286.419161561866131224.8326164426.84102564102564291.86697042142812325.8336832533.55128205128205280.70122529147183426.7024506140.26153846153846271.011127771101034527.4657283146.97179487179487262.497645720356455627.8603825753.68205128205127258.09573289551646727.7659980560.39230769230768259.14848333345547827.6472905167.1025641025641260.47252887749668927.2911127573.81282051282051264.445280869533591027.4430861380.52307692307691262.7501932179343101127.247722187.23333333333333264.92925345040123111227.4210708193.94358974358974262.9957486632421121327.48509571100.65384615384616262.28162479533813131427.40089284107.36410256410257263.22081066452114141527.47016555114.07435897435897262.4481534349712151627.28050201120.78461538461538264.56363139509665161727.35226625127.49487179487181263.7631841130806171827.26452993134.2051282051282264.74178158275873181927.41712097140.9153846153846263.0398045231192192027.38163031147.62564102564102263.43566191634466202127.29145662154.3358974358974264.4414453575215212227.23099654161.04615384615383265.1158078396257222327.21235602167.75641025641022265.3237213023065232427.04322916174.46666666666664267.2101362813738242527.08496417181.17692307692306266.7446304479759252626.71196582187.88717948717945270.9049966583484262726.63215716194.59743589743587271.7951701329526272826.50704941201.3076923076923273.190602760779282926.5331009208.0179487179487272.90002847155176293026.46867872214.72820512820508273.61858353270236303126.47112004221.43846153846152273.5913533476804313226.37258344228.14871794871792274.69041549676683323326.38536551234.85897435897434274.54784627930576333426.34906781241.56923076923073274.9527051480121343526.21192332248.27948717948712276.48239378587544353626.13201336254.98974358974363277.3736971077916363726.18836573261.7276.74515144128003373826.13277389268.4102564102564277.3652143308915383926.16587724275.12051282051283276.9959846065314394026.07131076281.83076923076925278.05076464916505404125.95063197288.54102564102567279.39679721160667414225.87795015295.25128205128203280.2074791120862424326.08289032301.96153846153845277.9216080015741434426.02287879308.6717948717949278.5909673805123444525.89566667315.3820512820513280.0098717964331454625.80753005322.09230769230766280.99293405563833464725.71269248328.8025641025641282.05073772720397474825.63386198335.5128205128205282.9300009739052484925.66854985342.2230769230769282.5430978021945495025.66205857348.93333333333334282.61550055523185505125.58376194355.6435897435897283.48880917515805515224.90347425362.3538461538462291.0766334173943525325.40748391369.06410256410254285.4549871206933535425.38268966375.77435897435896285.7315383643673545525.56880254382.4846153846154283.65566394011216555625.61887029389.19487179487174283.09721602012064565725.55484817395.90512820512816283.8113088363952575825.45589029402.61538461538464284.91506980475447585925.3297735409.325641025641286.321757115983596025.24410864416.0358974358974287.27724977537366606125.37182563422.74615384615385285.8527140757255616225.23402872429.45641025641027287.389679637544626325.15682984436.1666666666667288.2507440739231636425.16203024442.87692307692305288.1927396712025646525.20586406449.58717948717947287.70382393226214656625.1395952456.2974358974359288.44297664322666724.9573337463.0076923076923290.4758933086067676824.81200041469.7179487179487292.0969185525868686924.86125786476.4282051282051291.5475085301176697024.95156479483.1384615384615290.540238851568707124.91762126489.84871794871793290.9188397378109717224.85967434496.55897435897435291.5651707995919727324.81407953503.2692307692307292.07372826786826737424.85729753509.97948717948725291.59168140543983747524.71070555516.6897435897434293.22674580520476757624.31085515523.4297.68661560356423767724.6299896530.1102564102564294.1270390558382777824.52774075536.8205128205128295.26750701374993787924.32079302543.5307692307692297.5757701435124798024.3305757550.2410256410255297.4666556062191808124.41980041556.9512820512821296.47145693455974818224.29654763563.6615384615385297.84619951743315828324.48030061570.3717948717948295.79664703227957838424.44253771577.0820512820513296.2178486204541848524.13285025583.7923076923076299.67205492682564858624.15204919590.5025641025641299.4579129346597868724.09457507597.2128205128206300.0989703797024878823.90188741603.9230769230768302.24817883651707888923.99890803610.6333333333333301.1660257742664899024.09979644617.3435897435897300.0407319672814909124.11976223624.053846153846299.81803669597207919224.17371553630.7641025641026299.2162498785083929323.85724748637.4743589743589302.7460858206545939423.67680018644.1846153846153304.7587672597326949523.72771378650.8948717948718304.1908847669321959623.77033445657.6051282051282303.71550040278356969723.68460827664.3153846153846304.67167703926873979823.61178756671.0256410256409305.4839080042716989923.62344149677.7358974358974305.353921799999179910023.39568567684.4461538461538307.8942751991484710010123.32270312691.1564102564101308.708311378191210110223.39060016697.8666666666667307.950998245194610210323.42568326704.5769230769231307.559686747368310310423.33736979711.2871794871794308.5447216193637710410523.32866844717.9974358974359308.641775105118810510623.19068731724.7076923076922310.180795411118610610723.05266657731.4179487179487311.720257503109410710822.95958845738.1282051282051312.7584365413754310810922.84736563744.8384615384615314.010152601653610911022.78584056751.5487179487179314.696393751025211011122.71932009758.2589743589742315.438352815641111111222.61901678764.9692307692308316.557120497328811211322.50636943771.6794871794872317.81357178343611311422.58980955778.3897435897435316.8828935359682611411522.6023048785.1316.7435233480362611511622.12759651791.8102564102563322.038346579873211611722.33539634798.5205128205127319.720579289824311711822.27069308805.2307692307693320.4422694552777711811922.17465858811.9410256410255321.513423560151511912022.121931818.651282051282322.1015388454129612012121.81084352825.3615384615384325.571360758208812112221.84037639832.0717948717949325.241955695222812212321.87765295838.7820512820513324.8261786466025612312421.76106071845.4923076923076326.126630493995812412521.72953743852.2025641025641326.478236369044912512621.68473041858.9128205128205326.9780069397214712612721.51932633865.6230769230768328.8228986785561612712821.33760877872.3333333333334330.84974835573912812921.23556985879.0435897435897331.9878747971448512913021.13915422885.7538461538461333.0632799001615513013121.20694221892.4641025641026332.307183086397813113221.12037717899.1743589743588333.2727162335322613213321.06960093905.8846153846154333.8390665381002713313421.0880253912.5948717948718333.6335639481685613413520.80934976919.3051282051281336.741868053884113513620.82075819926.0153846153846336.6146201462819413613720.82642975932.7256410256409336.5513604746747713713820.56555741939.4358974358973339.4610903717600613813920.46371848946.1461538461538340.596986217527613914020.30875798952.8564102564102342.325391775761814014120.24266886959.5666666666666343.0625395920214414114220.20524973966.2769230769229343.4799068526782514214320.29263076972.9871794871794342.505272246991114314420.14127535979.6974358974359344.193467239874514414520.05955937986.4076923076923345.104914669837414514620.02357581993.1179487179487345.506269814347314614719.93067376999.8282051282051346.542485023824614714819.836064481006.5384615384614347.597742326554814814919.876094281013.2487179487179347.151256118270714915019.843308141019.9589743589743347.5169476808742415015119.213302911026.6692307692306354.54392913073036151top 1%top 5%meanbottom 5%bottom 1%

Read 2: Per position quality percentiles

Shows the mean for all bases and the means of the lowest and highest percentiles to indicate the spread. Since the graph is based on the sampled categories, rather than exact phreds, it is an approximation.

Per position quality percentiles001010202030304040505016111621263136414651566166717681869196101106111116121126131136141146151Per position quality percentilespositionphred score125.9153846420.13076923076923279.7899405330731226.5983035726.84102564102564272.17276791242862325.8605139833.55128205128205280.401959444578673426.5585597540.26153846153846272.61606436742164527.0148704546.97179487179487267.526444929695065627.382289453.68205128205127263.42831056815216727.1875086360.39230769230768265.600865234027657826.9901783167.1025641025641267.801857277558848927.0334838773.81282051282051267.3188337904086391026.5470533480.52307692307691272.7444050991022101126.6868981187.23333333333333271.18459800786917111226.8490393793.94358974358974269.3760993333043121327.06951304100.65384615384616266.91696993424455131426.77581037107.36410256410257270.1928843066897141526.53468848114.07435897435897272.8823208138241151626.66019487120.78461538461538271.4824418772736161726.61630709127.49487179487181271.97195934200465171826.56070547134.2051282051282272.59213133741576181926.20400942140.9153846153846276.5706641553512192026.58411815147.62564102564102272.3309898586931202126.70581663154.3358974358974270.97358372787136212226.40473131161.04615384615383274.33184306819874222326.50434568167.75641025641022273.22075974988024232426.48310248174.46666666666664273.4577031249258242526.3355482181.17692307692306275.1035008638679252626.33706531187.88717948717945275.08657928891364262726.1536948194.59743589743587277.1318657430546272826.24011264201.3076923076923276.16797438734096282926.22875613208.0179487179487276.29464317885265293026.11099185214.72820512820508277.6081677796565303126.10312523221.43846153846152277.6959109427728313226.11468641228.14871794871792277.5669592830667323325.93493784234.85897435897434279.5718472140677333426.00814962241.56923076923073278.7552542059053343525.82544944248.27948717948712280.7930639082405353625.96127367254.98974358974363279.2781013683146363725.70160495261.7282.17440629405894373825.68932328268.4102564102564282.3113942313438383925.77469082275.12051282051283281.3592178082139394025.63836227281.83076923076925282.87980544973396404125.59933785288.54102564102567283.3150778724808414225.40211973295.25128205128203285.5148184144773424325.45352746301.96153846153845284.94142442979444434425.39346186308.6717948717949285.61138690715654444525.50104252315.3820512820513284.4114488147065454625.39807762322.09230769230766285.5599034601227464725.38722957328.8025641025641285.6809009236518474825.33872356335.5128205128205286.2219295122655484925.40476878342.2230769230769285.485271276575495025.22044676348.93333333333334287.5411707296278505125.09241809355.6435897435897288.9691828124029515225.12191579362.3538461538462288.6401700120227525325.05782295369.06410256410254289.3550516592967535425.03068394375.77435897435896289.65775600437223545524.91552898382.4846153846154290.94217671703336555625.07358494389.19487179487174289.1792448448677565725.08571773395.90512820512816289.0439176295505575824.85218933402.61538461538464291.64865747831533585924.81170074409.325641025641292.10026096930994596024.97551856416.0358974358974290.2730622661184606124.86224166422.74615384615385291.5365352879836616224.79937434429.45641025641027292.23774778420943626324.75135066436.1666666666667292.77339648663553636424.72156984442.87692307692305293.10556712586623646524.77919156449.58717948717947292.462863385167656624.62791435456.2974358974359294.1501860641468666724.61053402463.0076923076923294.34404364295534676824.53332426469.7179487179487295.2052294503096686924.50183236476.4282051282051295.5564851915451697024.32728014483.1384615384615297.503413820607707124.24333385489.84871794871793298.4397378038112717224.32787315496.55897435897435297.4967994944198727324.20583648503.2692307692307298.857977763949737424.24758963509.97948717948725298.392269551696747524.20753638516.6897435897434298.83901731324744757624.06020553523.4300.4823229377788767724.02417581530.1102564102564300.88419286387506777823.94340337536.8205128205128301.7851163067472787923.95750299543.5307692307692301.6278513002545798023.94356935550.2410256410255301.7832648950241808123.98904302556.9512820512821301.27605863826176818223.96647563.6615384615385301.52783460265266828323.74448951570.3717948717948304.00377081524704838423.82663789577.0820512820513303.0875004279314848523.8059192583.7923076923076303.31859350613666858623.75274116590.5025641025641303.91173320435416868723.77717404597.2128205128206303.6392125994937878823.72117618603.9230769230768304.26380415301526888923.43944331610.6333333333333307.40620919795344899023.64186186617.3435897435897305.1484638359404909123.27373776624.053846153846309.2544633939318919223.66743615630.7641025641026304.8632122157129929323.71483647637.4743589743589304.33451629876646939423.5764576644.1846153846153305.87797289572995949523.23271247650.8948717948718309.71205320207685959623.31841885657.6051282051282308.75609740821477969723.36434535664.3153846153846308.2438403198546979823.24380146671.0256410256409309.5883683690525989922.97846666677.7358974358974312.5478718220699910023.1180622684.4461538461538310.9908447254589410010123.12492622691.1564102564101310.914284471372310110223.06340471697.8666666666667311.6004859592402510210322.94492871704.5769230769231312.9219489555942510310422.70305178711.2871794871794315.619807040089210410522.79285059717.9974358974359314.618204942818110510622.72657235724.7076923076922315.357462237760410610722.41966834731.4179487179487318.780622413975210710822.56295694738.1282051282051317.1824033780166610810922.28213835744.8384615384615320.31461066207910911022.52865036751.5487179487179317.5650536690154711011122.36473353758.2589743589742319.393356739038711111222.55814501764.9692307692308317.236074852266411211322.413634771.6794871794872318.847928463168311311422.39960093778.3897435897435319.0044511634842511411522.12535115785.1322.063390998772711511622.21978566791.8102564102563321.0100830665410311611722.13687806798.5205128205127321.934821681734511711822.21518994805.2307692307693321.0613429385479711811921.89621933811.9410256410255324.6190920580213511912022.12315871818.651282051282322.0878452132934612012122.17080812825.3615384615384321.556370927283212112221.77962452832.0717948717949325.9195726364621412212321.76069756838.7820512820513326.1306810641549412312421.74357422845.4923076923076326.321672172104212412521.79590757852.2025641025641325.7379540091592412512621.80299258858.9128205128205325.6589289249202512612721.63374324865.6230769230768327.5467099733890612712821.62783223872.3333333333334327.612640517286212812921.5189719879.0435897435897328.826851872859212913021.42614039885.7538461538461329.8622802841601413013121.01799378892.4641025641026334.414684739503613113221.16018033899.1743589743588332.82875789455413213321.17045449905.8846153846154332.714161408432613313421.23922078912.5948717948718331.9471528133790313413521.19349438919.3051282051281332.4571781137908613513621.02709833926.0153846153846334.313134044629713613720.84518841932.7256410256409336.342129273406113713820.88241147939.4358974358973335.9269490438167613813920.89880566946.1461538461538335.7440906843744413914020.78914618952.8564102564102336.967215719395314014120.61469424959.5666666666666338.913025777504114114220.50748727966.2769230769229340.108795780040914214320.37835715972.9871794871794341.5490933662174514314420.33954764979.6974358974359341.981968661981814414520.20601646986.4076923076923343.4713549029957614514620.29897673993.1179487179487342.434490305991314614720.37727085999.8282051282051341.5612097381232414714820.164286191006.5384615384614343.9368078511289514814920.163576161013.2487179487179343.94472748265314915020.042154981019.9589743589743345.299040581667515015119.190905331026.6692307692306354.7937482598468151top 1%top 5%meanbottom 5%bottom 1%

Read 1: Per sequence average quality scores

>=Q5 5,477,353 100.00%
>=Q7 5,477,353 100.00%
>=Q10 5,477,330 100.00%
>=Q12 5,477,310 100.00%
>=Q15 5,473,870 99.94%
>=Q20 5,214,420 95.20%
>=Q30 1,971,310 35.99%
Per sequence quality scores00112233445566778899101011111212131314141515036912151821242730333639035.080300187617254568.84615384615380061.656285178236395568.84615384615381088.23227016885554568.846153846153820114.80825515947467568.846153846153830141.38424015009383568.846153846153840167.9602251407129568.846153846153850194.53621013133207568.846153846153863.651398769e-05221.11219512195123568.845510113877570.0002738549077247.68818011257034568.841325854081880.0001095419631274.2641651031895568.84422264932590.0001643129446300.8401500938087568.8432570509106100.0002008269323327.4161350844279568.8426133186343110.002629007113353.9921200750469568.7998051222621120.01307200759380.5681050656661568.6156976912475130.04710304412407.1440900562853568.0157392097595140.1861483092433.72007504690436565.5644067017058150.4883928423460.29606003752355560.2359127848426160.910348484486.8720450281426552.7969426002131171.359287963513.4480300187618544.8822542634166181.792599455540.0240150093808537.2430833408657192.631508322566.5999999999999522.4533342934564203.571341851593.1759849906191505.8843092344238214.5385061619.7519699812382488.8334505665998225.346670189646.3279549718575474.58572409581365235.173192234672.9039399624764477.64409614039437247.199755064699.4799249530956441.9163110748766256.971524384726.0559099437148445.93995966773116269.788432478752.6318949343339396.27858948224923273.341851438779.2078799249531509.930166590809852810.64665724805.783864915572381.1483060606905292.548566799832.3598499061914523.91557215901413015.81680056858.9358348968104290.0310.005988293981885.5118198874295568.7405817528447320.148374589912.0878048780488566.2303477415119331.847388693938.6637898686678536.2771630603156344.983940235965.239774859287480.9805605283257358.170132544991.8157598499062424.80880396674047362.4690028191018.3917448405252525.31826478901663701044.9677298311444568.84615384615383801071.5437148217636568.846153846153839Per sequence quality scoresPhred scorePercentage of total

Read 2: Per sequence average quality scores

>=Q5 5,477,353 100.00%
>=Q7 5,477,350 100.00%
>=Q10 5,477,301 100.00%
>=Q12 5,477,227 100.00%
>=Q15 5,466,968 99.81%
>=Q20 5,243,046 95.72%
>=Q30 1,549,324 28.29%
Per sequence quality scores00112233445566778899101011111212036912151821242730333639035.080300187617254568.84615384615380061.656285178236395568.84615384615381088.23227016885554568.846153846153820114.80825515947467568.846153846153830141.38424015009383568.846153846153840167.9602251407129568.846153846153855.477098153e-05194.53621013133207568.844945188631867.302797537e-05221.11219512195123568.84454230279170.00023734092247.68818011257034568.840916330224680.000584223803274.2641651031895568.83326149925190.0005111958276300.8401500938087568.8348730426139100.0008398217168327.4161350844279568.827621097481110.01644955145353.9921200750469568.4831537036712120.04807066479380.5681050656661567.7853554275557130.1227782836407.1440900562853566.136746567357140.2459034501433.72007504690436563.4196844575868150.4208602221460.29606003752355559.5588294460314160.7163131535486.8720450281426553.0389278857684171.120431712513.4480300187618544.12104980158181.584634038540.0240150093808533.8772744156043192.390150863566.5999999999999516.101548237494203.125433033593.1759849906191499.87572388860747214.516707249619.7519699812382469.17380839705226225.799918318646.3279549718575440.85657419667893236.580149207672.9039399624764423.63884490795635249.607852552699.4799249530956356.8250628557482259.138373955726.0559099437148367.18527224969012611.78591192752.6318949343339308.76078205865434274.277412831779.2078799249531474.454433111281162810.21434989805.783864915572343.44119522725316293.049246598832.3598499061914501.556966501903963012.63606709858.9358348968104290.0310.007211512568885.5118198874295568.6870139390721320.1819126867912.0878048780488564.8317993292867331.854180295938.6637898686678527.9290678633135343.835447524965.239774859287484.20749354326813354.922724535991.8157598499062460.2140301854297361.7992084861018.3917448405252529.14215712970123701044.9677298311444568.84615384615383801071.5437148217636568.846153846153839Per sequence quality scoresPhred scorePercentage of total

Read 1: Per position base content

Base content000.10.10.20.20.30.30.40.40.50.50.60.60.70.70.80.80.90.91116111621263136414651566166717681869196101106111116121126131136141146151Base contentpositionfraction10.340441797721.446153846153848378.98438206974714120.126857340628.594871794871796498.09879080059955230.111575866535.743589743589745506.6211513760415340.0779339947642.892307692307696525.3829644608375450.0773766087450.04102564102564525.6938143577465560.170272392557.189743589743586473.8865503240202670.17865436164.33846153846154469.21199099975144780.178898822171.48717948717949469.0756568982247890.188152927278.63589743589743463.914713703161749100.119191514685.78461538461539502.3739630185490310110.223144829392.93333333333334444.399999064680211120.156486719100.08205128205128481.574714380917512130.2138465676107.23076923076924449.5855680812337613140.1544724249114.37948717948719482.69807072307214150.1645543021121.52820512820514477.0754853948332715160.1568249959128.6769230769231481.3860599984950616170.1468620073135.82564102564106486.942342103318117180.1519776661142.97435897435898484.089378510146918190.1599931573150.1230769230769479.6192007484131619200.1538067749157.27179487179487483.06929860519820210.1571350249164.4205128205128481.213159194568321220.1616972651171.56923076923078478.6688329338867322230.1528088476178.71794871794873483.6258349748360423240.1555866492185.86666666666665482.0766763828047524250.1597989029193.0153846153846479.7275349411125425260.1522074623200.16410256410254483.961222973662926270.1543585013207.3128205128205482.7616050616476327280.1602133366214.46153846153845479.496408420973828290.1534874601221.61025641025643483.2473780253401429300.1563903221228.75897435897434481.6284742058660430310.159835782235.90769230769232479.706967735355731320.1527732465243.05641025641023483.6456894556409332330.1559455817250.20512820512823481.876502489151533340.1607552042257.3538461538462479.194213041338734350.1529945213264.50256410256407483.522286221099935360.1557624641271.6512820512821481.9786257929837336370.1580482397278.8480.7038663074607737380.1515868352285.94871794871796484.307341927636938390.1547743956293.0974358974359482.529663998706639400.1594752913300.24615384615385479.908010612985240410.1529081227307.3948717948718483.5704700382515341420.1551639998314.54358974358973482.3123847062061642430.1593963361321.69230769230774479.95204330098343440.1529314637328.8410256410257483.5574529126857344450.155125295335.9897435897436482.333970090465845460.159844056343.13846153846157479.702353382136746470.1528504736350.2871794871795483.6026205049718747480.1545728833357.4358974358975482.6420458551156748490.1582211335364.58461538461546480.6074448032443349500.1509709161371.73333333333335484.650835273618850510.1541259845378.88205128205124482.891277881840651520.1587930031386.03076923076924480.2885175163357352530.1515453645393.1794871794872484.330469823533653540.15510576400.3282051282051482.3448646004459354550.1594823691407.4769230769231479.9040633950733655560.1529783005414.625641025641483.531332433853256570.1553266697421.774358974359482.2216650016054657580.1588296509428.92307692307696480.2680793119458590.1511266482436.0717948717949484.5639846473287759600.1530790511443.22051282051285483.475144556419660610.1578671783450.3692307692308480.8048428965296461620.1512024637457.51794871794874484.521702948515462630.1535176098464.6666666666667483.2305637538248763640.1585270249471.81538461538463480.436851481380364650.151100625478.96410256410263484.578497597235765660.154099477486.1128205128206482.9060608884273666670.1586520389493.2615384615385480.3671321617472467680.1509681775500.41025641025647484.65236254137368690.1550280612507.5589743589744482.3881966250635369700.1584336738514.7076923076924480.488912694140770710.1511553117521.8564102564103484.5479992448345671720.1537908913529.0051282051282483.0781567581724472730.1565891408536.1538461538462481.517594566909673740.150135221543.3025641025642485.1168959710668774750.1529231364550.4512820512821483.562097000559975760.1581634954557.6480.6395890837473576770.1504045841564.7487179487181484.966674245191677780.1534266923571.8974358974359483.281267747907878790.1571716669579.0461538461539481.192724208282679800.1502714906586.1948717948717485.0408994580467480810.1536299763593.3435897435897483.167897819164481820.1568439006600.4923076923077481.3755169537459482830.1499441427607.6410256410256485.223458863601383840.1523764472614.7897435897436483.8669813489342584850.1562911866621.9384615384616481.6837612985688785860.1492927604629.0871794871795485.5867297527373786870.1528769462636.2358974358975483.5878569166810687880.1559216623643.3846153846154481.8898421594501588890.1513574166650.5333333333333484.435286884573189900.1530145103657.6820512820514483.511138500344890910.157414722664.8307692307692481.057174247937291920.1507783453671.9794871794873484.758230523111292930.1532152483679.1282051282051483.399188440109793940.1570499537686.2769230769231481.260602734293194950.1513668047693.4256410256411484.430051220426795960.1537854275700.574358974359483.081203906716696970.1572184594707.723076923077481.166628436989797980.1511946194714.8717948717949484.526077618401898990.1544141502722.0205128205129482.7305700654868699100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Read 2: Per position base content

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Read 1: Per position N content

Per position N 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position N contentpositionfractionN

Read 2: Per position N content

Per position N content000.10.10.20.20.30.30.40.40.50.50.60.60.70.70.80.80.90.9111611162126313641465156616671768186919610110611111612112613113614114615111.588358464e-0524.996841569027568.8417247696666123.286258892e-0632.098217014773304568.8452374855012235.477098153e-0739.19959246051962568.846001119378334046.300967906265925568.8461538461538456.207377907e-0653.402343352012224568.8444229426991562.190839261e-0660.503718797758545568.8455429390522672.501208157e-0567.60509424350484568.8391793234096783.103688953e-0674.70646968925115568.8452883944265891.42404552e-0581.80784513499745568.84218294999289107.667937414e-0688.90922058074376568.844015671297910113.048917972e-0596.01059602649008568.837652055655311123.103688953e-06103.11197147223636568.845288394426512131.168447606e-05110.21334691798269568.842895674944913141.825699384e-07117.31472236372898568.846102937228714152.099554292e-05124.41609780947529568.840299319762615169.128496922e-07131.51747325522157568.845899301528116171.825699384e-05138.6188487009679568.841062953639717180145.7202241467142568.846153846153818195.659668092e-06152.82159959246047568.844575669474519200159.9229750382068568.846153846153820211.442302514e-05167.02435048395313568.842132041067821229.128496922e-07174.1257259296994568.845899301528122232.464694169e-05181.2271013754457568.839281141259823242.190839261e-06188.32847682119203568.845542939052224251.478816501e-05195.42985226693833568.842030223217425261.204961594e-05202.53122771268465568.842793857094526276.389947845e-06209.63260315843095568.844372033773927281.095419631e-06216.73397860417725568.84584839260328297.850507353e-06223.83535404992355568.843964762372729300230.93672949566988568.846153846153830311.058905643e-05238.03810494141615568.843201128495631323.286258892e-06245.13948038716248568.845237485501232332.921119015e-06252.2408558329088568.845339303351633341.460559507e-06259.3422312786551568.845746574752634355.84223803e-06266.4436067244015568.844524760549335362.775063064e-05273.54498217014776568.838415689532436370280.6463576158941568.846153846153837387.302797537e-07287.74773306164036568.845950210453338391.643129446e-06294.8491085073867568.845695665827639401.42404552e-05301.950483953133568.842182949992840410309.05185939887934568.846153846153841423.286258892e-06316.1532348446256568.845237485501242435.477098153e-07323.25461029037194568.846001119378343443.651398769e-07330.35598573611827568.846052028303644451.606615458e-05337.45736118186454568.841673860741445461.405788526e-05344.5587366276109568.84223385891846475.477098153e-07351.6601120733572568.846001119378347483.286258892e-06358.7614875191034568.845237485501248491.643129446e-06365.8628629648498568.845695665827649501.588358464e-05372.96423841059607568.841724769666650511.843956378e-05380.0656138563424568.841012044714651522.263867237e-05387.1669893020887568.839841139436452532.738549077e-06394.268364747835568.845390212276753548.033077291e-06401.3697401935813568.843913853447754550408.47111563932765568.846153846153855562.318638218e-05415.5724910850739568.83968841266156573.651398769e-07422.67386653082025568.846052028303657585.111958276e-06429.7752419765666568.844728396249858595.111958276e-06436.87661742231285568.844728396249859601.643129446e-06443.9779928680591568.845695665827660613.833968707e-06451.07936831380545568.845084758725961627.302797537e-07458.1807437595518568.845950210453362631.825699384e-05465.28211920529805568.841062953639763642.008269323e-06472.38349465104443568.845593847977364659.676206737e-06479.4848700967907568.843455673121365663.46882883e-06486.586245542537568.845186576576266672.61075012e-05493.68762098828336568.838873869858767680500.7889964340296568.846153846153868697.485367476e-06507.89037187977596568.844066580223169700514.9917473255222568.846153846153870718.945926983e-06522.0931227712683568.84365930882271723.286258892e-06529.1944982170147568.845237485501272736.572517784e-06536.295873662761568.844321124848873745.111958276e-06543.3972491085073568.844728396249874757.667937414e-06550.4986245542535568.844015671297975769.858776676e-06557.5999999999999568.843404764196376779.128496922e-07564.7013754457462568.845899301528177781.990012329e-05571.8027508914926568.840604773313578793.194973923e-05578.9041263372388568.837244784254279809.676206737e-06586.0055017829851568.843455673121380819.31106686e-06593.1068772287315568.843557490971681820600.2082526744778568.846153846153882831.332760551e-05607.309628120224568.842437494618583841.825699384e-07614.4110035659704568.846102937228784851.460559507e-05621.5123790117166568.842081132142585861.825699384e-06628.6137544574628568.845644756902486871.825699384e-06635.7151299032092568.845644756902487884.746818399e-06642.8165053489555568.844830214100288892.555979138e-06649.9178807947018568.845441121201989903.651398769e-07657.0192562404482568.846052028303690911.825699384e-07664.1206316861945568.846102937228791920671.2220071319407568.846153846153892930678.3233825776871568.846153846153893941.825699384e-07685.4247580234335568.846102937228794951.077162637e-05692.5261334691796568.843150219570695967.667937414e-06699.627508914926568.844015671297996971.734414415e-05706.7288843606724568.841317498265497984.929388338e-06713.8302598064186568.84477930517598999.128496922e-07720.9316352521649568.8458993015281991004.016538646e-06728.0330106979113568.84503384980081001015.84223803e-06735.1343861436576568.84452476054931011023.46882883e-06742.2357615894039568.84518657657621021032.921119015e-06749.3371370351502568.84533930335161031043.651398769e-07756.4385124808965568.84605202830361041051.296246563e-05763.5398879266428568.84253931246881051063.46882883e-06770.6412633723892568.84518657657621061071.42404552e-05777.7426388181354568.84218294999281071085.477098153e-06784.8440142638816568.84462657839961081099.128496922e-07791.945389709628568.84589930152811091103.651398769e-07799.0467651553743568.84605202830361101111.277989569e-06806.1481406011205568.84579748367791111129.128496922e-07813.2495160468669568.84589930152811121136.207377907e-06820.3508914926132568.84442294269911131142.190839261e-06827.4522669383596568.84554293905221141151.131933618e-05834.5536423841058568.84299749279511151161.643129446e-06841.6550178298521568.84569566582761161175.294528215e-06848.7563932755985568.84467748732481171181.277989569e-06855.8577687213448568.84579748367791181192.008269323e-06862.959144167091568.84559384797731191202.921119015e-06870.0605196128374568.84533930335161201219.493636799e-06877.1618950585837568.84350658204651211226.572517784e-06884.26327050433568.84432112484881221231.825699384e-06891.3646459500764568.84564475690241231247.120227599e-06898.4660213958227568.84416839807341241253.286258892e-06905.5673968415689568.84523748550121251261.570101471e-05912.6687722873153568.84177567859171261272.555979138e-06919.7701477330617568.84544112120191271285.111958276e-06926.8715231788077568.84472839624981281296.755087722e-06933.9728986245541568.84427021592361291301.570101471e-05941.0742740703005568.84177567859171301312.190839261e-06948.1756495160466568.84554293905221311323.103688953e-06955.277024961793568.84528839442651321334.929388338e-06962.3784004075394568.8447793051751331345.477098153e-07969.4797758532856568.84600111937831341353.651398769e-07976.581151299032568.84605202830361351361.825699384e-06983.6825267447783568.84564475690241361372.555979138e-06990.7839021905246568.84544112120191371381.460559507e-06997.8852776362709568.84574657475261381392.629007113e-051004.9866530820174568.83882296093361391403.46882883e-061012.0880285277635568.84518657657621401413.651398769e-071019.1894039735098568.84605202830361411421.004134661e-051026.290779419256568.84335385527111421431.643129446e-061033.3921548650026568.84569566582761431445.477098153e-071040.4935303107488568.84600111937831441451.277989569e-061047.594905756495568.84579748367791451465.477098153e-061054.6962812022416568.84462657839961461471.332760551e-051061.7976566479877568.84243749461851471484.016538646e-061068.899032093734568.84503384980081481498.580787106e-061076.0004075394802568.84376112667221491501.478816501e-051083.1017829852267568.84203022321741501518.398217168e-061090.203158430973568.8438120355974151Per position N contentpositionfractionN

Read 1: Per sequence GC content

For short reads with fixed size (i.e. Illumina) the plot will look very spiky due to the GC content calculation: GC bases / all bases. For read lengths of 151, both 75 and 76 GC bases lead to a percentage of 50% (rounded) and 72 and 73 GC bases leads to 48% (rounded). Only 74 GC bases leads to 49%. As a result the even categories will be twice as high, which creates a spike. The smoothened plot is provided to give a clearer picture in this case.

Per sequence GC content001000001000002000002000003000003000004000004000000369121518212427303336394245485154576063666972757881848790939699126.38035034272658568.84552202906530436.848743335872044568.84362657779981447.31713632901751568.84362657779982557.78552932216298568.84299476071123468.25392231530844568.843626577799841378.72231530845391568.837940224003152489.19070830159939568.830990236029565399.65910129474486568.8126675404624741110.12749428789033568.82024934552468154120.59588728103577568.74885401452229143131.06428027418124568.755804002495910499141.53267326732671568.530877118983811399152.00106626047216568.5940588278355121552162.46945925361763567.8655737247752131557172.93785224676313567.8624146393327145503183.40624523990857565.369264408044154894193.87463823305404565.75404101495091615152204.3430312261995559.27286132094221711433214.81142421934499561.62258907313731837825225.27981721249046544.94767247299311927400235.74821020563593551.53436562078422077814246.21660319878137519.68193892028132153862256.68499619192687534.815221824443722135751267.15338918507234483.07635226286382387854277.6217821782178513.338495351569124216114288.0901751713633432.30163557836125276134298.55856816450876394.3799739255623526157673309.0269611576542469.225658048391127360081319.4953541507997341.340824795814128198068329.9637471439451443.703406757741329423057340.4321401370906301.551511829358830219478350.90053313023606430.1762028925893431441340361.3689261233815289.9999999999999432219331371.837319116527430.2690800046013433425070382.30571210967247300.279664030173834202652392.7741051028179440.807157223978735369339403.2424980959634335.491462190322436167795413.7108910891089462.830405478420637302007424.17928408225436378.032970394358438131518434.6476770753998485.750833998556839223392445.1160700685453427.703270808133254093490455.58446306169077509.777574240686641153592466.0528560548362471.80410358662954261622476.5212490479817529.91232121755074396426486.9896420411272507.92255926880024436802497.45803503427265545.59402135454624556969507.9264280274181532.8521661304214622620518.3948210205637554.55445130389614736241528.863214013709545.94847074120424815041539.3316070068545559.34299301776754926019549.8552.40690502002645010883560.2683929931454561.97008847182185118197570.7367859862909557.3489782864075527807581.2051789794364563.91355783610065312841591.6735719725818560.7329906125053545124602.1419649657274565.6087230845919558429612.6103579588728563.520567607043563300623.0787509520183566.7611574540473575464633.5471439451636565.3939052744962582263644.0155369383092567.4163517748395593547654.4839299314546566.6050986331836601291664.9523229246001568.0304779848782611809675.4207159177455567.703196733026362630685.889108910891568.44810908038863833696.3575019040366568.319850211419164245706.8258948971819568.691358659467165318717.2942878903275568.64523601200546691727.7626808834729568.788658491098867106738.2310738766184568.77918123477096837748.6994668697638568.82277661387876958759.1678598629093568.80950845501987021769.6362528560547568.8328856872957135780.1046458492002568.82404024805587210790.5730388423457568.83983567526877333801.0414318354912568.82530388223287429811.5098248286366568.82783115058687515821.9782178217821568.83667658982617620832.4466108149276568.83351750438357714842.9150038080732568.83730840691467819853.3833968012185568.83414932147217910863.8517897943641568.83983567526878018874.3201827875095568.8347811385605817884.788575780655568.84173112653428215895.2569687738003568.8366765898261833905.7253617669459568.8442583948884847916.1937547600912568.8417311265342852926.6621477532367568.8448902119768862937.1305407463823568.8448902119768870947.5989337395276568.8461538461538883958.0673267326732568.8442583948884891968.5357197258186568.8455220290653900979.0041127189642568.8461538461538910989.4725057121095568.8461538461538920999.9408987052552568.84615384615389311010.4092916984005568.84552202906539411020.877684691546568.84552202906539501031.3460776846912568.84615384615389601041.8144706778367568.84615384615389701052.2828636709824568.84615384615389811062.7512566641278568.84552202906539911073.219649657273568.8455220290653100Per sequence GC contentGC %number of reads
Per sequence GC content (smoothened)0010000010000020000020000030000030000040000040000050000050000060000060000006121824303642485460667278849096Per sequence GC content (smoothened)GC %number of reads521.146153846153844568.84181622542630942.29230769230769568.838443355772121763.43846153846154568.831697616463947784.58461538461538568.78110457165136195105.73076923076923568.68160491685348642126.87692307692308568.304686733101951148.02307692307687567.2009151386734127060169.16923076923075562.89291737288661420046190.31538461538457551.94289604062851649258211.46153846153842527.310828956235718105214232.60769230769228480.12775536407220189613253.75384615384615408.961048878529522303968274.9312.534921552918824433807296.0461538461538203.052415796021726558149317.192307692307798.2050761613656428642535338.338461538461527.04933150219915230660671359.484615384615311.75674049019903632627722380.630769230769239.5399110489987634537134401.776923076923115.9252901069535836433525422.9230769230769203.2902031066405538316882444.0692307692307301.6456118744356440215214465.21538461538455387.373839874437342133228486.36153846153843456.50586274110274479589507.5076923076923501.73520158608924651282528.6538461538461525.60415691122644836902549.8537.72962331796235026004570.9461538461538546.91900669074455217965592.0923076923076553.69763147820975411729613.2384615384616558.9559352690584567727634.3846153846152562.330491358054584838655.5307692307691564.7665464657772602439676.676923076923566.7894250408648621078697.8230769230769567.937043940695664409718.9692307692306568.501156390355466143740.1153846153846568.72545222235766879761.2615384615383568.77941813682427045782.4076923076923568.80808752888477262803.553846153846568.79375283285457435824.6999999999999568.816519703027633845.8461538461538568.81820613784717828866.9923076923077568.82242222491498022888.1384615384613568.82748152939618210909.2846153846153568.8376001383587844930.4307692307691568.8426594428399863951.576923076923568.8435026602534881972.7230769230769568.8451890950805900993.8692307692307568.84603231249419221015.0153846153846568.8443458776679401036.1615384615384568.84603231249419611057.3076923076922568.84518909508059811078.453846153846568.8451890950805100

Read 2: Per sequence GC content

For short reads with fixed size (i.e. Illumina) the plot will look very spiky due to the GC content calculation: GC bases / all bases. For read lengths of 151, both 75 and 76 GC bases lead to a percentage of 50% (rounded) and 72 and 73 GC bases leads to 48% (rounded). Only 74 GC bases leads to 49%. As a result the even categories will be twice as high, which creates a spike. The smoothened plot is provided to give a clearer picture in this case.

Per sequence GC content0010000010000020000020000030000030000040000040000003691215182124273033363942454851545760636669727578818487909396991326.38035034272658568.837911313320104836.848743335872044568.815719878767813747.31713632901751568.822694329627229357.78552932216298568.787188034343435868.25392231530844568.8093794688957413078.72231530845391568.763728517816859389.19070830159939568.7871880343434622799.65910129474486568.70222654205767126110.12749428789033568.76626468176568328120.59588728103577568.63818840234979275131.06428027418124568.671792574671610777141.53267326732671568.353503999093311554152.00106626047216568.4948951392406122108162.46945925361763567.5095954451194131856172.93785224676313567.6693737738958146152183.40624523990857564.9455336928506155126193.87463823305404565.59605974572591616115204.3430312261995558.62858333729791712212214.81142421934499561.10324531037051838866225.27981721249046544.20351683733011927875235.74821020563593551.17226132773252079166246.21660319878137518.65166505285012153951256.68499619192687534.639008545298322136745267.15338918507234482.14421905040422388324277.6217821782178512.845117691999824216100288.0901751713633431.8298965105182725274892298.55856816450876394.553358790589126157486309.0269611576542468.993574934724427358647319.4953541507997341.449255906988228195596329.9637471439451444.830272912229829419694340.4321401370906302.7429557609051530217431350.90053313023606430.985987956544531439792361.3689261233815290.032217017371.837319116527431.248480925248633421457382.30571210967247301.625141500457634200668392.7741051028179441.6144170251111535366931403.2424980959634336.1968603689576336167170413.7108910891089462.8535220146027337299518424.17928408225436378.939465439606438131667434.6476770753998485.3638791834461639230214445.1160700685453422.88104201706544099406455.58446306169077505.818675471761541166415466.0528560548362463.33222295994474267865476.5212490479817525.81696224928743105502486.9896420411272501.95356161374194440221497.45803503427265543.34439129966344560625507.9264280274181530.40741899667254622000518.3948210205637554.89725212757914733798528.863214013709547.41683656049994813380539.3316070068545560.36268543731164921699549.8555.0880984647288508791560.2683929931454563.27229952760885114015570.7367859862909559.9600694104346525559581.2051789794364565.3215199982649539085591.6735719725818563.0858914773697543495602.1419649657274566.6301805958622555329612.6103579588728565.4673494253227562059623.0787509520183567.5406634534927573190633.5471439451636566.8235630969605581274644.0155369383092568.0383856284509591858654.4839299314546567.668105691921560596664.9523229246001568.46826541777861793675.4207159177455568.34335934329862244685.889108910891568.691447845275163359696.3575019040366568.618533131746264118706.8258948971819568.771337009663365218717.2942878903275568.70793291094246678727.7626808834729568.796698649151667162738.2310738766184568.74343920622626861748.6994668697638568.807477345934169132759.1678598629093568.76246043584247064769.6362528560547568.805575222972571118780.1046458492002568.77133700966337259790.5730388423457568.808745427908573115801.0414318354912568.773239132624974111811.5098248286366568.77577529657387551821.9782178217821568.813817755806276107832.4466108149276568.77831146052267744842.9150038080732568.81825604271677880853.3833968012185568.79543056717727940863.8517897943641568.82079220666558098874.3201827875095568.78401782940758143884.788575780655568.81889008370398292895.2569687738003568.78782207533078324905.7253617669459568.83093686246088483916.1937547600912568.79352844421568544926.6621477532367568.81825604271678688937.1305407463823568.79035823927968738947.5989337395276568.82206028863998878958.0673267326732568.79669864915168948968.5357197258186568.815719878767890109979.0041127189642568.77704337854819140989.4725057121095568.82079220666559294999.9408987052552568.786553993356393571010.4092916984005568.810013509883941031020.877684691546568.780847624471395831031.3460776846912568.7935284442156961961041.8144706778367568.7218818126611971101052.2828636709824568.776409337561983591062.7512566641278568.6185331317462999151073.219649657273568.2660063428586100Per sequence GC contentGC %number of reads
Per sequence GC content (smoothened)10000010000020000020000030000030000040000040000050000050000060000060000006121824303642485460667278849096Per sequence GC content (smoothened)GC %number of reads6121.146153846153844568.8461538461538013042.29230769230769568.7876208614583218863.43846153846154568.738419222149432084.58461538461538568.62644307751416454105.73076923076923568.51277032462781052126.87692307692308568.0054844572657102662148.02307692307687566.6397148143697128008169.16923076923075562.10468095665411421241190.31538461538457550.87907245700011651078211.46153846153842525.568222248870818107041232.60769230769228478.094578444281320190696253.75384615384615407.1296967818803422304424274.9310.653765137608824432378296.0461538461538202.109858996543126554243317.192307692307798.7312766788276728637125338.338461538461528.4221341058420230656809359.484615384615311.72411568921779732622125380.630769230769241.1466959961775334534101401.776923076923115.8178183851701436431185422.9230769230769203.121885818887838329620444.0692307692307289.279894378349340234280465.21538461538455370.157209753321642145723486.36153846153843445.280478241484164482625507.5076923076923498.80677198524334647178528.6538461538461528.87660834035814830490549.8543.03310759541935019574570.9461538461538552.29319543507955212580592.0923076923076558.2262344924799547388613.2384615384616562.630629514788564464634.3846153846152565.1110707792774582454655.5307692307691566.8161620725823601037676.676923076923568.018209019156162477697.8230769230769568.493259329728564296718.9692307692306568.646802376538666223740.1153846153846568.708728577738368196761.2615384615383568.731632789140870177782.4076923076923568.747750567535372226803.553846153846568.706183665360274158824.6999999999999568.763868345929776124845.8461538461538568.792710686214578138866.9923076923077568.780834428450180135888.1384615384613568.783379340828282107909.2846153846153568.807131856356884132930.4307692307691568.785924253206386116951.576923076923568.799497119222688157972.7230769230769568.764716650055790134993.8692307692307568.7842276449542921601015.0153846153846568.7621717376776942791036.1615384615384568.661223546681964691057.3076923076922568.5000457627367989151078.453846153846568.1217021225308100

Adapter Content

By calculating the overlap between reads it is possible to determine where the adapter is.

Total reads 5,477,353 100.00%
Adapters in read 1 1,834,674 33.50%
Adapters in read 2 1,834,674 33.50%
Longest most frequent adapter Adapter SequenceBest match
Read 1 CTGTCTCTTATACACATCTCCGAGCCCACGA gnl|uv|NGB00726.1:1-34 Illumina Nextera transposase sequence Read 2 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
Read 2 CTGTCTCTTATACACATCTGACGCTGCCGAC gnl|uv|NGB00725.1:1-33 Illumina Nextera transposase sequence Read 1 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)

Adapters read 1

AdapterCount
C 21908
CT 22392
CTG 22566
CTGT 22406
CTGTC 22114
CTGTCT 22459
CTGTCTC 22067
CTGTCTCT 22882
CTGTCTCTT 22742
CTGTCTCTTA 22790
CTGTCTCTTAT 22434
CTGTCTCTTATA 22582
CTGTCTCTTATAC 22132
CTGTCTCTTATACA 22417
CTGTCTCTTATACAC 22842
CTGTCTCTTATACACA 22801
CTGTCTCTTATACACAT 22547
CTGTCTCTTATACACATC 22387
CTGTCTCTTATACACATCT 22785
CTGTCTCTTATACACATCTC 22647
CTGTCTCTTATACACATCTCC 22655
CTGTCTCTTATACACATCTCCG 21916
CTGTCTCTTATACACATCTCCGA 21661
CTGTCTCTTATACACATCTCCGAG 22071
CTGTCTCTTATACACATCTCCGAGC 22229
CTGTCTCTTATACACATCTCCGAGCC 22234
CTGTCTCTTATACACATCTCCGAGCCC 22728
CTGTCTCTTATACACATCTCCGAGCCCA 21942
CTGTCTCTTATACACATCTCCGAGCCCAC 22272
CTGTCTCTTATACACATCTCCGAGCCCACG 22093
CTGTCTCTTATACACATCTCCGAGCCCACGA 1049249

Adapters read 2

AdapterCount
C 21351
CT 22003
CTG 22202
CTGT 22198
CTGTC 21694
CTGTCT 22182
CTGTCTC 21657
CTGTCTCT 22528
CTGTCTCTT 22440
CTGTCTCTTA 22510
CTGTCTCTTAT 22274
CTGTCTCTTATA 22421
CTGTCTCTTATAC 21408
CTGTCTCTTATACA 21996
CTGTCTCTTATACAC 22437
CTGTCTCTTATACACA 22327
CTGTCTCTTATACACAT 22121
CTGTCTCTTATACACATC 22027
CTGTCTCTTATACACATCT 22470
CTGTCTCTTATACACATCTG 22118
CTGTCTCTTATACACATCTGA 22460
CTGTCTCTTATACACATCTGAC 22209
CTGTCTCTTATACACATCTGACG 21792
CTGTCTCTTATACACATCTGACGC 21918
CTGTCTCTTATACACATCTGACGCT 22235
CTGTCTCTTATACACATCTGACGCTG 22269
CTGTCTCTTATACACATCTGACGCTGC 22964
CTGTCTCTTATACACATCTGACGCTGCC 22048
CTGTCTCTTATACACATCTGACGCTGCCG 22280
CTGTCTCTTATACACATCTGACGCTGCCGA 22053
CTGTCTCTTATACACATCTGACGCTGCCGAC 1063178

Insert Sizes

Insert sizes are calculated by taking the first and last 16 bp from read 2. These are searched for in read 1 while allowing at most 1 error. This method is very fast and reasonably accurate, but does not account for long poly-G cycles.

Total reads 5,477,353
Reads without overlap 1,552,343 28.34%
Insert Sizes0020002000400040006000600080008000100001000012000120001400014000160001600018000180002000020000220002200024000240000102030405060708090100110120130140150160170180190200210220230240250260270280023.163763066202087568.84615384615380026.87590458322165568.84615384615381030.588046100241222568.84615384615382034.30018761726078568.84615384615383038.01232913428035568.84615384615384041.72447065129991568.84615384615385045.43661216831948568.84615384615386049.148753685339045568.84615384615387052.86089520235861568.84615384615388056.57303671937817568.84615384615389060.28517823639774568.846153846153810063.99731975341731568.846153846153811067.70946127043689568.846153846153812071.42160278745645568.846153846153813075.13374430447602568.846153846153814078.84588582149559568.8461538461538151382.55802733851515568.700507174933616986.27016885553472568.745321535309117589.98231037255428568.790135895684418993.69445188957384568.745321535309119397.4065934065934568.81254307587222010101.11873492361298568.7341179452152218104.83087644063254568.756525125403226108.5430179576521568.77893230559062311112.25515947467169568.7229143551214246115.96730099169125568.77893230559062517119.67944250871082568.65569281455822654123.39158402573037568.24115998108532753127.10372554274996568.25236357117912858130.8158670597695568.196345620709729102134.52800857678903567.703387656579830160138.24015009380858567.053579431135731163141.95229161082815567.019968660854132179145.66443312784773566.840711219352433214149.37657464486728566.448585566067134307153.08871616188685565.406651687337935365156.80085767890643564.756843461893836530160.51299919592597562.908251096406537649164.22514071294555561.575023875236938749167.93728222996512560.454664865850539783171.64942374698467560.073742802659140901175.36156526400424558.7517191715833411041179.07370678102382557.1832165584426421061182.78584829804336556.9591447565654431265186.49798981506294554.6736123774173441458190.21013133208248552.5113194893017451666193.9222728491021550.1809727497782461800197.63441436612163548.6796916772006471945201.3465558831412547.0551711135904482360205.05869740016078542.4056812246374492543208.77083891718036540.3554242374605502708212.4829804341999538.5068318719731513106216.19512195121948534.0478030146156523185219.90726346823905533.1627193972005533598223.61940498525863528.535636688435544141227.33154650227817522.4520872674675554257231.04368801929775521.1524708165795564708234.75582953631732516.0996516842473575254238.46797105333687509.9824914929981585520242.1801125703564507.0023365280306596085245.89225408737602500.67230812499804606293249.6043956043956498.3419613854746616411253.31653712141514497.0199377543987627090257.0286786384347489.41270008066584637362260.7408201554543486.3653235751351647869264.45296167247386480.68510339754664658504268.16510318949344473.5708236879437669147271.877244706513466.3669152575898679818275.5893862235326458.84930630460786810160279.30152774055216455.017678492506666910930283.01366925757173446.390914120232257010841286.72581077459125447.38803363858617111435290.4379522916109440.73310112283157211739294.15009380863046437.32720973429727311994297.86223532565434.470294260362157412253301.57437684266955431.568564426051647513075305.2865183596891422.359213368896357613588308.99865987670876416.61177165074477714292312.7108013937282408.724444224665157814859316.42294291074785402.37200864144497915116320.1350844277674399.492685987322158015740323.84722594478694392.50164576875178116128327.5593674618065388.154652812332878216054331.27150897882615388.983718479278768316534334.9836504958456383.605995234224578416873338.69579201286524379.8079781924058517158342.4079335298848376.61495501565418617581346.12007504690433371.87583640595018718549349.8322165639239361.03076119509088818643353.5443580809435359.977623726267678919406357.256499597963351.429284484650349019724360.96864111498263347.866542834801869119691364.68078263200215348.23626130789949219883368.3929241490217346.08517200987779320242372.1050656660413342.063083166180959420215375.8172071830609342.36558009871529520055379.5293487000805344.15815451373339620875383.2414902171334.97121063676579721058386.9536317341196332.92095364958889821516390.66577325113917327.789709386599559922616394.37791476815875315.465760283350410022072398.09005628517826321.560513294411810122561401.8021978021979316.081957738512810222849405.5143393192174312.855323791480310322698409.226480836237314.547065895653610422679412.93862235325656314.75993410743710523280416.6507638702761308.026576461025410622761420.36290538729565313.8412397197402610722663424.0750469043153314.939191548938810823736427.78718842133475302.917739378223910923611431.4993299383544304.3181881399567611023620435.21147145537395304.21735582911211124407438.92361297239347295.400130425241911224089442.63575448941305298.9628720750903311323945446.3478960064327300.576189048606611423946450.06003752345214300.564985458512711523981453.77217904047177300.172859805227511623500457.48432055749134305.5617866403755411723877461.1964620745109301.338033174989211824235464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SizesInsert sizeNumber of reads

Read 1: Per tile quality

This graph shows the deviation of each tile on each position from the geometric mean of all tiles at that position. The scale is expressed in phred units. -10 is 10 times more errors than the average. -3 is ~2 times more errors than the average. Only points that deviate more than 2 phred units from the average are shown.

Tiles with more than 2 times the average error: 1142, 1549, 1569, 2113, 2136, 2150, 2231, 2256, 2264, 2273, 2548, 2550, 2619, 2671, 2676

Tiles with more than 10 times the average error: 2114, 2610, 2611, 2617, 2618

Deviation from geometric mean in phred units.-20-20-18-18-16-16-14-14-12-12-10-10-8-8-6-6-4-4-2-2002216111621263136414651566166717681869196101106111116121126131136141146151Deviation from geometric mean in phred units.positionNormalized phred16-4.437713539107.63076923076923199.769779284939261625-4.303686234161.44615384615383196.605577723877272528-3.859666322179.38461538461536186.12287417205012876-6.624963174466.4251.407755087028657676-17.56960363466.4509.79576436195794762-3.03283356323.91794871794872166.60248230227558228-8.292811761179.38461538461536290.78338173783612876-4.949208374466.4211.845473564816877632-5.941743406203.30256410256408235.277869739161732116-4.136448688705.5794871794872192.65732772349561164-3.08357782535.87692307692307167.80048499893405453-6.746115629328.8717948717949254.26799906031977536-3.64401737147.83589743589743181.031697058998766116-16.06521289705.5794871794872474.27915422698925116116-16.22732132705.5794871794872478.106312825494411652-19.07082372322.8923076923077545.23751997804495252-19.06786312322.8923076923077545.16762423618145252-3.990428479322.8923076923077189.20999006460488524-3.49420632335.87692307692307177.49486287033238429-3.022920328185.36410256410255166.36844436959626292 times more errors10 times more errors11421549156921132114213621502231225622642273254825502610261126172618261926712676

Read 2: Per tile quality

This graph shows the deviation of each tile on each position from the geometric mean of all tiles at that position. The scale is expressed in phred units. -10 is 10 times more errors than the average. -3 is ~2 times more errors than the average. Only points that deviate more than 2 phred units from the average are shown.

Tiles with more than 2 times the average error: 1444, 1540, 2278, 2373, 2669

Tiles with more than 10 times the average error:

Deviation from geometric mean in phred units.-11-11-10-10-9-9-8-8-7-7-6-6-5-5-4-4-3-3-2-2-1-10011223316111621263136414651566166717681869196101106111116121126131136141146151Deviation from geometric mean in phred units.positionNormalized phred43-3.507085384269.0769230769231276.949576874131434-3.00251686835.87692307692307257.29343101218586445-3.856966782281.0358974358974290.57967780233744541-3.229189209257.1179487179487266.1237572298347412-4.28868111423.91794871794872307.3976908399195622 times more errors10 times more errors14441540227823732669

Duplication percentages

Fingerprints are taken by taking a sample from the beginning and the end at an offset. The samples are combined and hashed while using the length as a seed. A subsample of these fingerprints is stored to estimate the duplication rate. See the documentation for a complete explanation.

Fingerprint front sequence length 8
Fingerprint front sequence offset 0
Fingerprint back sequence length 8
Fingerprint back sequence offset 0
Subsampled fingerprints 572,140
Estimated remaining sequences if deduplicated 83.47%
Duplication levels (%)001010202030304040505060607070123456-1011-2021-3031-5051-100101-500501-10001001-50005001-1000010001-50000> 5000070.7195063253.60864984393376264.3316667091815120.30752622118.98505209263345445.511723273847925.673771281184.36145434133314498.1052643883947431.796139875249.73785659003286512.041425517142240.70316717315.1142588387325515.96955641167150.7286970983380.4906610874322515.87780215292266-100.06725312559445.86706333613193518.255023919579611-200.003938903234511.24346558483165518.482574481275521-300576.6198678335314518.496730852625231-500641.9962700822311518.496730852625251-1000707.3726723309309518.4967308526252101-5000772.7490745796306518.4967308526252501-10000838.1254768283303518.49673085262521001-50000903.50187907703518.49673085262525001-100000968.8782813257296518.496730852625210001-5000001034.2546835744292518.4967308526252> 50000Duplication levels (%)Duplication countsPercentage of total

Read 1: Overrepresented sequences

A subsample of the sequences is analysed Sequences are cut into fragments of up to 31 bp. Fragments are stored and counted. When the maximum amount of unique fragments is reached, only fragments that are already stored are counted. The rest of the fragments is ignored. Fragments are stored in their canonical representation. That is either the sequence or the reverse complement, whichever has the lowest sort order. Both representations are shown in the table. See the documentation for a complete explanation. The sequence identity is calculated by Number of matched nucleotides - gap sequences in query alignment Length of query .

The percentage shown is an estimate based on the number of occurences of the fragment in relation to the number of sampled sequences. Fragments are only counted once per sequence. Fragments that occur more than once in a sequence are counted as one.

Total sequences in file 5,477,353
Sampled sequences 684,670
Sampling rate 1 in 8
Total fragments sampled 5,475,263
Stored unique fragments 1,828,940
Maximum unique fragments 5,000,000
Fragment size 21
countpercentagecanonical sequencereverse complemented sequencesequence identitybest match
9890 1.44% CTGTCTCTTATACACATCTCC GGAGATGTGTATAAGAGACAG 100.00% gnl|uv|NGB00726.1:1-34 Illumina Nextera transposase sequence Read 2 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
9597 1.40% CGGAGATGTGTATAAGAGACA TGTCTCTTATACACATCTCCG 100.00% gnl|uv|NGB00726.1:1-34 Illumina Nextera transposase sequence Read 2 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
9317 1.36% GTCTCTTATACACATCTCCGA TCGGAGATGTGTATAAGAGAC 100.00% gnl|uv|NGB00726.1:1-34 Illumina Nextera transposase sequence Read 2 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
9240 1.35% CTCTTATACACATCTCCGAGC GCTCGGAGATGTGTATAAGAG 100.00% gnl|uv|NGB00726.1:1-34 Illumina Nextera transposase sequence Read 2 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
9239 1.35% CTCGGAGATGTGTATAAGAGA TCTCTTATACACATCTCCGAG 100.00% gnl|uv|NGB00726.1:1-34 Illumina Nextera transposase sequence Read 2 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
8908 1.30% CTTATACACATCTCCGAGCCC GGGCTCGGAGATGTGTATAAG 100.00% gnl|uv|NGB00726.1:1-34 Illumina Nextera transposase sequence Read 2 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
8905 1.30% GGCTCGGAGATGTGTATAAGA TCTTATACACATCTCCGAGCC 100.00% gnl|uv|NGB00726.1:1-34 Illumina Nextera transposase sequence Read 2 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
8597 1.26% TGGGCTCGGAGATGTGTATAA TTATACACATCTCCGAGCCCA 100.00% gnl|uv|NGB00726.1:1-34 Illumina Nextera transposase sequence Read 2 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
8394 1.23% GTGGGCTCGGAGATGTGTATA TATACACATCTCCGAGCCCAC 100.00% gnl|uv|NGB00726.1:1-34 Illumina Nextera transposase sequence Read 2 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
8230 1.20% ATACACATCTCCGAGCCCACG CGTGGGCTCGGAGATGTGTAT 100.00% gnl|uv|NGB00726.1:1-34 Illumina Nextera transposase sequence Read 2 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
7983 1.17% TACACATCTCCGAGCCCACGA TCGTGGGCTCGGAGATGTGTA 100.00% gnl|uv|NGB00726.1:1-34 Illumina Nextera transposase sequence Read 2 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
7841 1.15% ACACATCTCCGAGCCCACGAG CTCGTGGGCTCGGAGATGTGT 100.00% gnl|uv|NGB00726.1:1-34 Illumina Nextera transposase sequence Read 2 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
7650 1.12% CACATCTCCGAGCCCACGAGA TCTCGTGGGCTCGGAGATGTG 100.00% gnl|uv|NGB00726.1:1-34 Illumina Nextera transposase sequence Read 2 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
7463 1.09% ACATCTCCGAGCCCACGAGAC GTCTCGTGGGCTCGGAGATGT 100.00% gnl|uv|NGB00726.1:1-34 Illumina Nextera transposase sequence Read 2 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
7425 1.08% AGTCTCGTGGGCTCGGAGATG CATCTCCGAGCCCACGAGACT 95.24% gnl|uv|NGB00726.1:1-34 Illumina Nextera transposase sequence Read 2 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
7205 1.05% AAGTCTCGTGGGCTCGGAGAT ATCTCCGAGCCCACGAGACTT 90.48% gnl|uv|NGB00726.1:1-34 Illumina Nextera transposase sequence Read 2 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
7087 1.04% CAAGTCTCGTGGGCTCGGAGA TCTCCGAGCCCACGAGACTTG 85.71% gnl|uv|NGB00726.1:1-34 Illumina Nextera transposase sequence Read 2 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
7016 1.02% CACAAGTCTCGTGGGCTCGGA TCCGAGCCCACGAGACTTGTG 85.71% gnl|uv|NGB00740.1:1-47 Illumina Nextera PCR primer i7 index N706 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
6896 1.01% ACAAGTCTCGTGGGCTCGGAG CTCCGAGCCCACGAGACTTGT 80.95% gnl|uv|NGB00726.1:1-34 Illumina Nextera transposase sequence Read 2 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
6765 0.99% CCGAGCCCACGAGACTTGTGA TCACAAGTCTCGTGGGCTCGG 90.48% gnl|uv|NGB00738.1:1-47 Illumina Nextera PCR primer i7 index N704 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
6601 0.96% CGAGCCCACGAGACTTGTGAA TTCACAAGTCTCGTGGGCTCG 85.71% gnl|uv|NGB00738.1:1-47 Illumina Nextera PCR primer i7 index N704 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
6423 0.94% CTTCACAAGTCTCGTGGGCTC GAGCCCACGAGACTTGTGAAG 85.71% gnl|uv|NGB00738.1:1-47 Illumina Nextera PCR primer i7 index N704 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
6255 0.91% AGCCCACGAGACTTGTGAAGG CCTTCACAAGTCTCGTGGGCT 80.95% gnl|uv|NGB00738.1:1-47 Illumina Nextera PCR primer i7 index N704 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
6222 0.91% CCCCCCCCCCCCCCCCCCCCC GGGGGGGGGGGGGGGGGGGGG 100.00% Poly-G. Illumina dark cycle pattern.
6042 0.88% GCCCACGAGACTTGTGAAGGC GCCTTCACAAGTCTCGTGGGC 80.95% gnl|uv|NGB00738.1:1-47 Illumina Nextera PCR primer i7 index N704 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
5900 0.86% CCCACGAGACTTGTGAAGGCA TGCCTTCACAAGTCTCGTGGG 80.95% gnl|uv|U59231.1:5103-7490 Cloning vector cLHYGpk
5692 0.83% ATGCCTTCACAAGTCTCGTGG CCACGAGACTTGTGAAGGCAT 80.95% gnl|uv|U59231.1:5103-7490 Cloning vector cLHYGpk
5446 0.80% CACGAGACTTGTGAAGGCATC GATGCCTTCACAAGTCTCGTG 80.95% gnl|uv|U59231.1:5103-7490 Cloning vector cLHYGpk
3895 0.57% CGAGACTTGTGAAGGCATCTC GAGATGCCTTCACAAGTCTCG 80.95% gnl|uv|U59231.1:5103-7490 Cloning vector cLHYGpk
3719 0.54% CGAGATGCCTTCACAAGTCTC GAGACTTGTGAAGGCATCTCG 80.95% gnl|uv|U59231.1:5103-7490 Cloning vector cLHYGpk
3580 0.52% ACGAGATGCCTTCACAAGTCT AGACTTGTGAAGGCATCTCGT 80.95% gnl|uv|U59231.1:5103-7490 Cloning vector cLHYGpk
3496 0.51% ACGAGACTTGTGAAGGCATCT AGATGCCTTCACAAGTCTCGT 76.19% gnl|uv|U59231.1:5103-7490 Cloning vector cLHYGpk
3378 0.49% CCTGTCTCTTATACACATCTC GAGATGTGTATAAGAGACAGG 100.00% gnl|uv|JN835257.1:94-190 Cloning vector pIGWZMCS
3371 0.49% GACTTGTGAAGGCATCTCGTA TACGAGATGCCTTCACAAGTC 80.95% gnl|uv|U59231.1:5103-7490 Cloning vector cLHYGpk
3287 0.48% ACTTGTGAAGGCATCTCGTAT ATACGAGATGCCTTCACAAGT 80.95% gnl|uv|NGB00366.1:1-43 Illumina Multiplexing PCR Primer Index 3 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
3110 0.45% CATACGAGATGCCTTCACAAG CTTGTGAAGGCATCTCGTATG 71.43% gnl|uv|NGB01090.1:1-66 Rubicon Genomics ThruPLEX DNA-seq dual-index D502
3024 0.44% GAGATGTGTATAAGAGACAGA TCTGTCTCTTATACACATCTC 100.00% gnl|uv|JN243340.1:723-820 Cloning vector R995 Km Tp
2934 0.43% GCATACGAGATGCCTTCACAA TTGTGAAGGCATCTCGTATGC 76.19% gnl|uv|NGB01090.1:1-66 Rubicon Genomics ThruPLEX DNA-seq dual-index D502
2876 0.42% GGCATACGAGATGCCTTCACA TGTGAAGGCATCTCGTATGCC 80.95% gnl|uv|NGB01090.1:1-66 Rubicon Genomics ThruPLEX DNA-seq dual-index D502
2815 0.41% CGGCATACGAGATGCCTTCAC GTGAAGGCATCTCGTATGCCG 85.71% gnl|uv|NGB01090.1:1-66 Rubicon Genomics ThruPLEX DNA-seq dual-index D502
2510 0.37% ACGGCATACGAGATGCCTTCA TGAAGGCATCTCGTATGCCGT 90.48% gnl|uv|NGB01090.1:1-66 Rubicon Genomics ThruPLEX DNA-seq dual-index D502
2492 0.36% AAGGCATCTCGTATGCCGTCT AGACGGCATACGAGATGCCTT 95.24% gnl|uv|NGB00366.1:1-43 Illumina Multiplexing PCR Primer Index 3 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
2405 0.35% GAAGGCATCTCGTATGCCGTC GACGGCATACGAGATGCCTTC 90.48% gnl|uv|NGB00366.1:1-43 Illumina Multiplexing PCR Primer Index 3 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
2298 0.34% AAGACGGCATACGAGATGCCT AGGCATCTCGTATGCCGTCTT 100.00% gnl|uv|NGB00366.1:1-43 Illumina Multiplexing PCR Primer Index 3 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
2150 0.31% GAAGACGGCATACGAGATGCC GGCATCTCGTATGCCGTCTTC 100.00% gnl|uv|NGB00366.1:1-43 Illumina Multiplexing PCR Primer Index 3 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
2076 0.30% GAGATGTGTATAAGAGACAGC GCTGTCTCTTATACACATCTC 95.24% gnl|uv|NGB00726.1:1-34 Illumina Nextera transposase sequence Read 2 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
2004 0.29% ACGAGACTTGTGAAGGCATCG CGATGCCTTCACAAGTCTCGT 80.95% gnl|uv|JQ437370.1:1-703 Nanoluc luciferase reporter vector pNL1.1[Nluc]
1903 0.28% AGAAGACGGCATACGAGATGC GCATCTCGTATGCCGTCTTCT 100.00% gnl|uv|NGB00366.1:1-43 Illumina Multiplexing PCR Primer Index 3 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
1902 0.28% CAGAAGACGGCATACGAGATG CATCTCGTATGCCGTCTTCTG 100.00% gnl|uv|NGB00374.1:1-43 Illumina Multiplexing PCR Primer Index 11 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
1703 0.25% ATCTCGTATGCCGTCTTCTGC GCAGAAGACGGCATACGAGAT 100.00% gnl|uv|NGB00380.1:1-26 Illumina Small RNA 3' Adapter (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
1672 0.24% CGTATGCCGTCTTCTGCTTGA TCAAGCAGAAGACGGCATACG 100.00% gnl|uv|JX069761.1:1-102 Cloning vector pFosill-1
1649 0.24% ACTGTCTCTTATACACATCTC GAGATGTGTATAAGAGACAGT 100.00% gnl|uv|JN243349.1:63-159 Cloning vector R995 Tp
1570 0.23% AGCAGAAGACGGCATACGAGA TCTCGTATGCCGTCTTCTGCT 100.00% gnl|uv|NGB00380.1:1-26 Illumina Small RNA 3' Adapter (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
1532 0.22% AGATGTGTATAAGAGACAGAT ATCTGTCTCTTATACACATCT 100.00% gnl|uv|NGB00399.1:2658-2791 pCDC-1K Cloning Vector
1526 0.22% GTATGCCGTCTTCTGCTTGAA TTCAAGCAGAAGACGGCATAC 95.24% gnl|uv|JX069761.1:1-102 Cloning vector pFosill-1
1454 0.21% CGAGACTTGTGAAGGCATCGC GCGATGCCTTCACAAGTCTCG 80.95% gnl|uv|JQ437370.1:1-703 Nanoluc luciferase reporter vector pNL1.1[Nluc]
1383 0.20% AAGCAGAAGACGGCATACGAG CTCGTATGCCGTCTTCTGCTT 100.00% gnl|uv|NGB00380.1:1-26 Illumina Small RNA 3' Adapter (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
1370 0.20% ACGCGATGCCTTCACAAGTCT AGACTTGTGAAGGCATCGCGT 80.95% gnl|uv|U59231.1:5103-7490 Cloning vector cLHYGpk
1366 0.20% CGCGATGCCTTCACAAGTCTC GAGACTTGTGAAGGCATCGCG 80.95% gnl|uv|U59231.1:5103-7490 Cloning vector cLHYGpk
1330 0.19% TATGCCGTCTTCTGCTTGAAA TTTCAAGCAGAAGACGGCATA 95.24% gnl|uv|JX069761.1:1-102 Cloning vector pFosill-1
1328 0.19% CAAGCAGAAGACGGCATACGA TCGTATGCCGTCTTCTGCTTG 100.00% gnl|uv|NGB00379.1:1-23 Illumina 3' RNA Adapter (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
1243 0.18% ATGCCGTCTTCTGCTTGAAAA TTTTCAAGCAGAAGACGGCAT 90.48% gnl|uv|JX069761.1:1-102 Cloning vector pFosill-1
1222 0.18% ACCTGTCTCTTATACACATCT AGATGTGTATAAGAGACAGGT 100.00% gnl|uv|JN835257.1:94-190 Cloning vector pIGWZMCS
1186 0.17% GACTTGTGAAGGCATCGCGTA TACGCGATGCCTTCACAAGTC 80.95% gnl|uv|U59231.1:5103-7490 Cloning vector cLHYGpk
1154 0.17% ACTTGTGAAGGCATCGCGTAT ATACGCGATGCCTTCACAAGT 80.95% gnl|uv|U59231.1:5103-7490 Cloning vector cLHYGpk
1125 0.16% CATACGCGATGCCTTCACAAG CTTGTGAAGGCATCGCGTATG 76.19% gnl|uv|JQ437370.1:1-703 Nanoluc luciferase reporter vector pNL1.1[Nluc]
1097 0.16% CGGCATACGCGATGCCTTCAC GTGAAGGCATCGCGTATGCCG 76.19% gnl|uv|X66730.1:1-2687-49 B.bronchiseptica plasmid pBBR1 genes for mobilization and replication
1055 0.15% GCATACGCGATGCCTTCACAA TTGTGAAGGCATCGCGTATGC 76.19% gnl|uv|JQ437370.1:1-703 Nanoluc luciferase reporter vector pNL1.1[Nluc]
1046 0.15% GGCATACGCGATGCCTTCACA TGTGAAGGCATCGCGTATGCC 76.19% gnl|uv|JQ437370.1:1-703 Nanoluc luciferase reporter vector pNL1.1[Nluc]
1038 0.15% GAAGGCATCGCGTATGCCGTC GACGGCATACGCGATGCCTTC 80.95% gnl|uv|X66730.1:1-2687-49 B.bronchiseptica plasmid pBBR1 genes for mobilization and replication
1022 0.15% AGATGTGTATAAGAGACAGCT AGCTGTCTCTTATACACATCT 100.00% gnl|uv|AB777652.1:4182-4357 Cloning vector pRMTn-Gm DNA
960 0.14% ACGGCATACGCGATGCCTTCA TGAAGGCATCGCGTATGCCGT 76.19% gnl|uv|X66730.1:1-2687-49 B.bronchiseptica plasmid pBBR1 genes for mobilization and replication
954 0.14% AAGGCATCGCGTATGCCGTCT AGACGGCATACGCGATGCCTT 76.19% gnl|uv|X66730.1:1-2687-49 B.bronchiseptica plasmid pBBR1 genes for mobilization and replication
926 0.14% CTTTTCAAGCAGAAGACGGCA TGCCGTCTTCTGCTTGAAAAG 85.71% gnl|uv|JX069761.1:1-102 Cloning vector pFosill-1
811 0.12% CCTTTTCAAGCAGAAGACGGC GCCGTCTTCTGCTTGAAAAGG 80.95% gnl|uv|JX069761.1:1-102 Cloning vector pFosill-1
796 0.12% AGATGTGTATAAGAGACAGGA TCCTGTCTCTTATACACATCT 95.24% gnl|uv|JN835257.1:94-190 Cloning vector pIGWZMCS
759 0.11% AAGACGGCATACGCGATGCCT AGGCATCGCGTATGCCGTCTT 95.24% gnl|uv|NGB00366.1:1-43 Illumina Multiplexing PCR Primer Index 3 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
759 0.11% AACTGTCTCTTATACACATCT AGATGTGTATAAGAGACAGTT 95.24% gnl|uv|NGB00401.1:1458-1518 pPDM-2 Cloning Vector
752 0.11% AGATGTGTATAAGAGACAGGC GCCTGTCTCTTATACACATCT 95.24% gnl|uv|JN835257.1:94-190 Cloning vector pIGWZMCS
745 0.11% CCCTTTTCAAGCAGAAGACGG CCGTCTTCTGCTTGAAAAGGG 76.19% gnl|uv|JX069761.1:1-102 Cloning vector pFosill-1
721 0.11% CCCCTTTTCAAGCAGAAGACG CGTCTTCTGCTTGAAAAGGGG 71.43% gnl|uv|JX069761.1:1-102 Cloning vector pFosill-1
719 0.11% GAAGACGGCATACGCGATGCC GGCATCGCGTATGCCGTCTTC 95.24% gnl|uv|NGB00366.1:1-43 Illumina Multiplexing PCR Primer Index 3 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)

Read 2: Overrepresented sequences

A subsample of the sequences is analysed Sequences are cut into fragments of up to 31 bp. Fragments are stored and counted. When the maximum amount of unique fragments is reached, only fragments that are already stored are counted. The rest of the fragments is ignored. Fragments are stored in their canonical representation. That is either the sequence or the reverse complement, whichever has the lowest sort order. Both representations are shown in the table. See the documentation for a complete explanation. The sequence identity is calculated by Number of matched nucleotides - gap sequences in query alignment Length of query .

The percentage shown is an estimate based on the number of occurences of the fragment in relation to the number of sampled sequences. Fragments are only counted once per sequence. Fragments that occur more than once in a sequence are counted as one.

Total sequences in file 5,477,353
Sampled sequences 684,670
Sampling rate 1 in 8
Total fragments sampled 5,476,939
Stored unique fragments 1,885,977
Maximum unique fragments 5,000,000
Fragment size 21
countpercentagecanonical sequencereverse complemented sequencesequence identitybest match
9949 1.45% CTGTCTCTTATACACATCTGA TCAGATGTGTATAAGAGACAG 100.00% gnl|uv|NGB00725.1:1-33 Illumina Nextera transposase sequence Read 1 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
9722 1.42% GTCAGATGTGTATAAGAGACA TGTCTCTTATACACATCTGAC 100.00% gnl|uv|NGB00725.1:1-33 Illumina Nextera transposase sequence Read 1 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
9443 1.38% CGTCAGATGTGTATAAGAGAC GTCTCTTATACACATCTGACG 100.00% gnl|uv|NGB00725.1:1-33 Illumina Nextera transposase sequence Read 1 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
9375 1.37% GCGTCAGATGTGTATAAGAGA TCTCTTATACACATCTGACGC 100.00% gnl|uv|NGB00725.1:1-33 Illumina Nextera transposase sequence Read 1 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
9372 1.37% AGCGTCAGATGTGTATAAGAG CTCTTATACACATCTGACGCT 100.00% gnl|uv|NGB00725.1:1-33 Illumina Nextera transposase sequence Read 1 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
9046 1.32% CTTATACACATCTGACGCTGC GCAGCGTCAGATGTGTATAAG 100.00% gnl|uv|NGB00725.1:1-33 Illumina Nextera transposase sequence Read 1 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
9004 1.32% CAGCGTCAGATGTGTATAAGA TCTTATACACATCTGACGCTG 100.00% gnl|uv|NGB00725.1:1-33 Illumina Nextera transposase sequence Read 1 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
8663 1.27% GGCAGCGTCAGATGTGTATAA TTATACACATCTGACGCTGCC 100.00% gnl|uv|NGB00725.1:1-33 Illumina Nextera transposase sequence Read 1 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
8481 1.24% CGGCAGCGTCAGATGTGTATA TATACACATCTGACGCTGCCG 100.00% gnl|uv|NGB00725.1:1-33 Illumina Nextera transposase sequence Read 1 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
8372 1.22% ATACACATCTGACGCTGCCGA TCGGCAGCGTCAGATGTGTAT 100.00% gnl|uv|NGB00725.1:1-33 Illumina Nextera transposase sequence Read 1 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
8108 1.18% GTCGGCAGCGTCAGATGTGTA TACACATCTGACGCTGCCGAC 100.00% gnl|uv|NGB00725.1:1-33 Illumina Nextera transposase sequence Read 1 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
7912 1.16% ACACATCTGACGCTGCCGACG CGTCGGCAGCGTCAGATGTGT 100.00% gnl|uv|NGB00725.1:1-33 Illumina Nextera transposase sequence Read 1 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
7734 1.13% CACATCTGACGCTGCCGACGA TCGTCGGCAGCGTCAGATGTG 100.00% gnl|uv|NGB00725.1:1-33 Illumina Nextera transposase sequence Read 1 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
7512 1.10% ACATCTGACGCTGCCGACGAT ATCGTCGGCAGCGTCAGATGT 95.24% gnl|uv|NGB00725.1:1-33 Illumina Nextera transposase sequence Read 1 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
7496 1.09% CATCTGACGCTGCCGACGATC GATCGTCGGCAGCGTCAGATG 90.48% gnl|uv|NGB00725.1:1-33 Illumina Nextera transposase sequence Read 1 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
7352 1.07% ATCTGACGCTGCCGACGATCG CGATCGTCGGCAGCGTCAGAT 85.71% gnl|uv|NGB00725.1:1-33 Illumina Nextera transposase sequence Read 1 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
7156 1.05% ACGATCGTCGGCAGCGTCAGA TCTGACGCTGCCGACGATCGT 80.95% gnl|uv|NGB00725.1:1-33 Illumina Nextera transposase sequence Read 1 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
7074 1.03% TGACGATCGTCGGCAGCGTCA TGACGCTGCCGACGATCGTCA 80.95% gnl|uv|NGB00730.1:1-51 Illumina Nextera PCR primer i5 index N504 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
6988 1.02% CTGACGCTGCCGACGATCGTC GACGATCGTCGGCAGCGTCAG 80.95% gnl|uv|NGB00730.1:1-51 Illumina Nextera PCR primer i5 index N504 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
6845 1.00% CTGACGATCGTCGGCAGCGTC GACGCTGCCGACGATCGTCAG 85.71% gnl|uv|NGB00732.1:1-51 Illumina Nextera PCR primer i5 index N506 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
6683 0.98% ACGCTGCCGACGATCGTCAGT ACTGACGATCGTCGGCAGCGT 85.71% gnl|uv|NGB00732.1:1-51 Illumina Nextera PCR primer i5 index N506 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
6377 0.93% CGCTGCCGACGATCGTCAGTA TACTGACGATCGTCGGCAGCG 80.95% gnl|uv|NGB00732.1:1-51 Illumina Nextera PCR primer i5 index N506 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
6261 0.91% GCTGCCGACGATCGTCAGTAC GTACTGACGATCGTCGGCAGC 71.43% gnl|uv|NGB00730.1:1-51 Illumina Nextera PCR primer i5 index N504 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
6019 0.88% CGTACTGACGATCGTCGGCAG CTGCCGACGATCGTCAGTACG 80.95% gnl|uv|KY031735.1:1-4680 Cloning vector pABC4
5961 0.87% ACGTACTGACGATCGTCGGCA TGCCGACGATCGTCAGTACGT 76.19% gnl|uv|JN874480.1:31317-31418 Cloning vector pHUGE-Red
5891 0.86% CACGTACTGACGATCGTCGGC GCCGACGATCGTCAGTACGTG 80.95% gnl|uv|NGB01096.1:1-66 Rubicon Genomics ThruPLEX DNA-seq dual-index D508
5591 0.82% CGACGATCGTCAGTACGTGTA TACACGTACTGACGATCGTCG 76.19% gnl|uv|NGB00754.1:1-70 Illumina TruSeq DNA HT and RNA HT i5 index D508 adapter (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
5579 0.81% ACACGTACTGACGATCGTCGG CCGACGATCGTCAGTACGTGT 80.95% gnl|uv|JN874480.1:31317-31418 Cloning vector pHUGE-Red
5452 0.80% CTACACGTACTGACGATCGTC GACGATCGTCAGTACGTGTAG 80.95% gnl|uv|NGB00754.1:1-70 Illumina TruSeq DNA HT and RNA HT i5 index D508 adapter (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
5203 0.76% ACGATCGTCAGTACGTGTAGA TCTACACGTACTGACGATCGT 80.95% gnl|uv|NGB00754.1:1-70 Illumina TruSeq DNA HT and RNA HT i5 index D508 adapter (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
5183 0.76% ATCTACACGTACTGACGATCG CGATCGTCAGTACGTGTAGAT 85.71% gnl|uv|NGB00754.1:1-70 Illumina TruSeq DNA HT and RNA HT i5 index D508 adapter (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
4850 0.71% GATCGTCAGTACGTGTAGATC GATCTACACGTACTGACGATC 90.48% gnl|uv|NGB00754.1:1-70 Illumina TruSeq DNA HT and RNA HT i5 index D508 adapter (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
4691 0.69% AGATCTACACGTACTGACGAT ATCGTCAGTACGTGTAGATCT 90.48% gnl|uv|NGB00754.1:1-70 Illumina TruSeq DNA HT and RNA HT i5 index D508 adapter (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
4559 0.67% GAGATCTACACGTACTGACGA TCGTCAGTACGTGTAGATCTC 95.24% gnl|uv|NGB00754.1:1-70 Illumina TruSeq DNA HT and RNA HT i5 index D508 adapter (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
4378 0.64% CGAGATCTACACGTACTGACG CGTCAGTACGTGTAGATCTCG 95.24% gnl|uv|NGB00754.1:1-70 Illumina TruSeq DNA HT and RNA HT i5 index D508 adapter (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
4323 0.63% CCGAGATCTACACGTACTGAC GTCAGTACGTGTAGATCTCGG 100.00% gnl|uv|NGB00754.1:1-70 Illumina TruSeq DNA HT and RNA HT i5 index D508 adapter (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
4274 0.62% ACCGAGATCTACACGTACTGA TCAGTACGTGTAGATCTCGGT 100.00% gnl|uv|NGB00754.1:1-70 Illumina TruSeq DNA HT and RNA HT i5 index D508 adapter (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
4075 0.60% CACCGAGATCTACACGTACTG CAGTACGTGTAGATCTCGGTG 100.00% gnl|uv|NGB00754.1:1-70 Illumina TruSeq DNA HT and RNA HT i5 index D508 adapter (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
4010 0.59% AGTACGTGTAGATCTCGGTGG CCACCGAGATCTACACGTACT 100.00% gnl|uv|NGB00754.1:1-70 Illumina TruSeq DNA HT and RNA HT i5 index D508 adapter (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
3968 0.58% ACCACCGAGATCTACACGTAC GTACGTGTAGATCTCGGTGGT 100.00% gnl|uv|NGB00754.1:1-70 Illumina TruSeq DNA HT and RNA HT i5 index D508 adapter (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
3830 0.56% GACCACCGAGATCTACACGTA TACGTGTAGATCTCGGTGGTC 100.00% gnl|uv|NGB00731.1:1-51 Illumina Nextera PCR primer i5 index N505 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
3817 0.56% CCCCCCCCCCCCCCCCCCCCC GGGGGGGGGGGGGGGGGGGGG 100.00% Poly-G. Illumina dark cycle pattern.
3697 0.54% ACGTGTAGATCTCGGTGGTCG CGACCACCGAGATCTACACGT 100.00% gnl|uv|NGB00793.1:1-50 Illumina TruSeq Small RNA Sample Prep Kit RNA PCR Primer (RP1) (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
3500 0.51% CGTGTAGATCTCGGTGGTCGC GCGACCACCGAGATCTACACG 100.00% gnl|uv|NGB00844.1:1-48 Epicentre Biotechnologies Nextera DNA Sample Prep Kit Adaptor (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
3494 0.51% CAGATGTGTATAAGAGACAGG CCTGTCTCTTATACACATCTG 95.24% gnl|uv|NGB00725.1:1-33 Illumina Nextera transposase sequence Read 1 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
3363 0.49% GGCGACCACCGAGATCTACAC GTGTAGATCTCGGTGGTCGCC 100.00% gnl|uv|NGB00844.1:1-48 Epicentre Biotechnologies Nextera DNA Sample Prep Kit Adaptor (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
3265 0.48% CGGCGACCACCGAGATCTACA TGTAGATCTCGGTGGTCGCCG 100.00% gnl|uv|NGB00844.1:1-48 Epicentre Biotechnologies Nextera DNA Sample Prep Kit Adaptor (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
3048 0.45% CAGATGTGTATAAGAGACAGA TCTGTCTCTTATACACATCTG 95.24% gnl|uv|NGB00725.1:1-33 Illumina Nextera transposase sequence Read 1 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
2951 0.43% ACGGCGACCACCGAGATCTAC GTAGATCTCGGTGGTCGCCGT 100.00% gnl|uv|NGB00844.1:1-48 Epicentre Biotechnologies Nextera DNA Sample Prep Kit Adaptor (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
2819 0.41% TACGGCGACCACCGAGATCTA TAGATCTCGGTGGTCGCCGTA 100.00% gnl|uv|NGB00844.1:1-48 Epicentre Biotechnologies Nextera DNA Sample Prep Kit Adaptor (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
2485 0.36% GATACGGCGACCACCGAGATC GATCTCGGTGGTCGCCGTATC 100.00% gnl|uv|NGB00844.1:1-48 Epicentre Biotechnologies Nextera DNA Sample Prep Kit Adaptor (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
2449 0.36% AGATCTCGGTGGTCGCCGTAT ATACGGCGACCACCGAGATCT 100.00% gnl|uv|NGB00844.1:1-48 Epicentre Biotechnologies Nextera DNA Sample Prep Kit Adaptor (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
2367 0.35% ATCTCGGTGGTCGCCGTATCA TGATACGGCGACCACCGAGAT 100.00% gnl|uv|NGB00844.1:1-48 Epicentre Biotechnologies Nextera DNA Sample Prep Kit Adaptor (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
2216 0.32% ATGATACGGCGACCACCGAGA TCTCGGTGGTCGCCGTATCAT 100.00% gnl|uv|NGB00844.1:1-48 Epicentre Biotechnologies Nextera DNA Sample Prep Kit Adaptor (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
2185 0.32% AATGATACGGCGACCACCGAG CTCGGTGGTCGCCGTATCATT 100.00% gnl|uv|NGB00844.1:1-48 Epicentre Biotechnologies Nextera DNA Sample Prep Kit Adaptor (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
2089 0.31% CAGATGTGTATAAGAGACAGC GCTGTCTCTTATACACATCTG 100.00% gnl|uv|AB777652.1:5733-7306-49 Cloning vector pRMTn-Gm DNA
2014 0.29% TAATGATACGGCGACCACCGA TCGGTGGTCGCCGTATCATTA 100.00% gnl|uv|JX069762.1:9774-9902-49 Cloning vector pFosill-2
1832 0.27% CGGTGGTCGCCGTATCATTAA TTAATGATACGGCGACCACCG 100.00% gnl|uv|JX069762.1:9774-9902-49 Cloning vector pFosill-2
1765 0.26% GGTGGTCGCCGTATCATTAAA TTTAATGATACGGCGACCACC 95.24% gnl|uv|JX069762.1:9774-9902-49 Cloning vector pFosill-2
1689 0.25% GTGGTCGCCGTATCATTAAAA TTTTAATGATACGGCGACCAC 90.48% gnl|uv|JX069762.1:9774-9902-49 Cloning vector pFosill-2
1550 0.23% AGATGTGTATAAGAGACAGAT ATCTGTCTCTTATACACATCT 100.00% gnl|uv|NGB00399.1:2658-2791 pCDC-1K Cloning Vector
1522 0.22% ACTGTCTCTTATACACATCTG CAGATGTGTATAAGAGACAGT 95.24% gnl|uv|NGB00725.1:1-33 Illumina Nextera transposase sequence Read 1 (Oligonucleotide sequence copyright 2007-2012 Illumina, Inc. All rights reserved.)
1424 0.21% TGGTCGCCGTATCATTAAAAA TTTTTAATGATACGGCGACCA 85.71% gnl|uv|JX069762.1:9774-9902-49 Cloning vector pFosill-2
1295 0.19% ACCTGTCTCTTATACACATCT AGATGTGTATAAGAGACAGGT 100.00% gnl|uv|JN835257.1:94-190 Cloning vector pIGWZMCS
1080 0.16% AGATGTGTATAAGAGACAGCT AGCTGTCTCTTATACACATCT 100.00% gnl|uv|AB777652.1:4182-4357 Cloning vector pRMTn-Gm DNA
855 0.12% CTTTTTAATGATACGGCGACC GGTCGCCGTATCATTAAAAAG 80.95% gnl|uv|JX069762.1:9774-9902-49 Cloning vector pFosill-2
844 0.12% AGATGTGTATAAGAGACAGGA TCCTGTCTCTTATACACATCT 95.24% gnl|uv|JN835257.1:94-190 Cloning vector pIGWZMCS
773 0.11% AGATGTGTATAAGAGACAGGC GCCTGTCTCTTATACACATCT 95.24% gnl|uv|JN835257.1:94-190 Cloning vector pIGWZMCS
722 0.11% AACTGTCTCTTATACACATCT AGATGTGTATAAGAGACAGTT 95.24% gnl|uv|NGB00401.1:1458-1518 pPDM-2 Cloning Vector
699 0.10% CCCCTTTTTAATGATACGGCG CGCCGTATCATTAAAAAGGGG 66.67% gnl|uv|JX069762.1:9774-9902-49 Cloning vector pFosill-2
693 0.10% CCCTTTTTAATGATACGGCGA TCGCCGTATCATTAAAAAGGG 71.43% gnl|uv|JX069762.1:9774-9902-49 Cloning vector pFosill-2

Nanopore time series

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